Western Blot (WB)
(Western Blot analysis of Hela, KB, 293T, 3T3 cells with Phospho-FAK (Ser843) Polyclonal Antibody at dilution of 1:1000)
Rabbit FAK Polyclonal Antibody | anti-FAK antibody
Phospho-FAK (Ser843) Polyclonal Antibody
ELISA: 1:40000
Western Blot (WB)
(Western Blot analysis of Hela, KB, 293T, 3T3 cells with Phospho-FAK (Ser843) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of Hela, KB, 293T, 3T3 cells with Phospho-FAK (Ser843) Polyclonal Antibody at dilution of 1:1000)
NCBI and Uniprot Product Information
Observed Molecular Weight: 120kDa
NCBI Description
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
Uniprot Description
FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.
Protein type: EC 2.7.10.2; Fak family; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: apical plasma membrane; cell cortex; cytoplasm; cytoskeleton; cytosol; extrinsic component of cytoplasmic side of plasma membrane; focal adhesion; lamellipodium; microtubule organizing center; nucleus; plasma membrane; stress fiber
Molecular Function: actin binding; ATP binding; JUN kinase binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein kinase binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding; SH2 domain binding; signal transducer activity
Biological Process: angiogenesis; axon guidance; cell motility; central nervous system neuron axonogenesis; endothelial cell migration; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; establishment of cell polarity; extracellular matrix organization; Fc-gamma receptor signaling pathway involved in phagocytosis; heart morphogenesis; innate immune response; integrin-mediated signaling pathway; MAPK cascade; microtubule cytoskeleton organization; negative regulation of anoikis; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of cell-cell adhesion; negative regulation of organ growth; negative regulation of synaptogenesis; netrin-activated signaling pathway; neuron migration; nuclear migration; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; placenta development; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; protein autophosphorylation; regulation of cell adhesion mediated by integrin; regulation of cell proliferation; regulation of cell shape; regulation of cytoskeleton organization; regulation of endothelial cell migration; regulation of epithelial cell migration; regulation of focal adhesion formation; regulation of GTPase activity; regulation of osteoblast differentiation; regulation of protein amino acid phosphorylation; regulation of substrate adhesion-dependent cell spreading; signal complex assembly; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculogenesis
Research Articles on FAK
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Product Notes
The FAK ptk2 (Catalog #AAA2548829) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-FAK (Ser843) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FAK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:40000. Researchers should empirically determine the suitability of the FAK ptk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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