Western Blot (WB)
((0.5ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat FAIM1 Polyclonal Antibody | anti-FAIM antibody
Goat anti-FAIM1 Antibody
Gene Names
FAIM; FAIM1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
FAIM1; Polyclonal Antibody; Goat anti-FAIM1 Antibody; FAIM; Fas apoptotic inhibitory molecule; FAIM1 antibody; FAIM antibody; Fas apoptotic inhibitory molecule antibody; anti-FAIM antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KKYMEDRSKTTN
Sequence Length
213
Applicable Applications for anti-FAIM antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Placenta shows cytoplasm staining of trophoblasts. Recommended concentration, 2-3ug/ml.
Western Blot: Approx 25kDa band observed in Human Lymph Node lysates (calculated MW of 24. 0kDa according to NP_001028202.1). Recommended concentration: 0.5-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Placenta shows cytoplasm staining of trophoblasts. Recommended concentration, 2-3ug/ml.
Western Blot: Approx 25kDa band observed in Human Lymph Node lysates (calculated MW of 24. 0kDa according to NP_001028202.1). Recommended concentration: 0.5-1ug/ml.
Immunogen
Peptide with sequence C-KKYMEDRSKTTN, from the internal region of the protein sequence according to NP_001028202.1; NP_001028203.1; NP_001028204.1; NP_060617.1.
Epitope
Internal region
Note
This antibody is expected to recognise all reported isoforms (NP_001028202.1; NP_001028203.1; NP_001028204.1; NP_060617.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,626 Da
NCBI Official Full Name
fas apoptotic inhibitory molecule 1 isoform a
NCBI Official Synonym Full Names
Fas apoptotic inhibitory molecule
NCBI Official Symbol
FAIM
NCBI Official Synonym Symbols
FAIM1
NCBI Protein Information
fas apoptotic inhibitory molecule 1
UniProt Protein Name
Fas apoptotic inhibitory molecule 1
Protein Family
UniProt Gene Name
FAIM
UniProt Synonym Gene Names
FAIM1
UniProt Entry Name
FAIM1_HUMAN
NCBI Description
The protein encoded by this gene protects against death receptor-triggered apoptosis and regulates B-cell signaling and differentiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Uniprot Description
FAIM: Plays a role as an inducible effector molecule that mediates Fas resistance produced by surface Ig engagement in B cells. Belongs to the FAIM1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 3q22.3
Cellular Component: cytoplasm
Molecular Function: protein binding
Biological Process: apoptosis; negative regulation of apoptosis
Research Articles on FAIM
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Product Notes
The FAIM faim (Catalog #AAA420362) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FAIM1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's FAIM1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Placenta shows cytoplasm staining of trophoblasts. Recommended concentration, 2-3ug/ml. Western Blot: Approx 25kDa band observed in Human Lymph Node lysates (calculated MW of 24. 0kDa according to NP_001028202.1). Recommended concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the FAIM faim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KKYMEDRSKT TN. It is sometimes possible for the material contained within the vial of "FAIM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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