Testing Data
(All lanes: MBS641344 (1:1000) Lane 1: H. liver lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 60kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human FADS2 Polyclonal Antibody | anti-FADS2 antibody
FADS2, NT (FADS2, Fatty acid desaturase 2, Delta(6) fatty acid desaturase) (MaxLight 650)
Gene Names
FADS2; D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
FADS2; Polyclonal Antibody; NT (FADS2; Fatty acid desaturase 2; Delta(6) fatty acid desaturase) (MaxLight 650); Delta(6) fatty acid desaturase; anti-FADS2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FADS2.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Applicable Applications for anti-FADS2 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 79-108 in the N-terminal region of human FADS2. Species sequence homology: monkey
Conjugate
MaxLight650
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(All lanes: MBS641344 (1:1000) Lane 1: H. liver lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 60kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes: MBS641344 (1:1000) Lane 1: H. liver lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 60kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS641344. This demonstrates that MBS641344 detected the FADS2 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS641344. This demonstrates that MBS641344 detected the FADS2 protein (arrow).)
Product Categories/Family for anti-FADS2 antibody
References
1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Mathias, R.A., et al. J. Lipid Res. 51(9):2766-2774(2010). 3. Lu, Y., et al. Am. J. Clin. Nutr. 92(1):258-265(2010). 4. Zabaneh, D., et al. PLoS ONE 5 (8), E11961 (2010). 5. Steer, C.D., et al. PLoS ONE 5 (7), E11570 (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,259 Da
NCBI Official Full Name
fatty acid desaturase 2
NCBI Official Synonym Full Names
fatty acid desaturase 2
NCBI Official Symbol
FADS2
NCBI Official Synonym Symbols
D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262
NCBI Protein Information
fatty acid desaturase 2; OTTHUMP00000224924; OTTHUMP00000224926; OTTHUMP00000224929; OTTHUMP00000224931; delta-6 desaturase; delta-6-desaturase; delta(6) desaturase; delta-6 fatty acid desaturase; delta(6) fatty acid desaturase; linoleoyl-CoA desaturase (
UniProt Protein Name
Fatty acid desaturase 2
Protein Family
UniProt Gene Name
FADS2
UniProt Synonym Gene Names
D6D; Delta(6) desaturase; Delta-6 desaturase
Uniprot Description
Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
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Product Notes
The FADS2 fads2 (Catalog #AAA6297622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FADS2, NT (FADS2, Fatty acid desaturase 2, Delta(6) fatty acid desaturase) (MaxLight 650) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FADS2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FADS2 fads2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FADS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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