Goat Factor XI Polyclonal Antibody

Goat anti-human Factor XI (F.XI), Whole IgG from antiserum

Cat. Num. AAA512063

• Purity: Purified IgG.; Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 5 mg.
• Synonyms: Factor XI; Polyclonal Antibody; Goat anti-human Factor XI (F.XI); Whole IgG from antiserum; human; anti-Factor XI antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: This antibody is specific for F.XI as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Purified IgG.; Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 5 mg.
• Form/Format: Whole IgG, clear liquid.
• Concentration: IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (varies by lot)

• Application Notes: Suitable as a source of antibodies to human F.XI.
• Immunogen: Human Factor XI purified from plasma.
• Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
• Neutralizing Activity: XXXX Bethesda Units/ml IgG against normal plasma. One Bethesda unit/ml is defined as the amount of inhibitor that resulted in 50% residual F.XI activity after 2 hours at 37 degree C (Kasper CK et al, Thromb Diath Haemorrh 34:869, 1975).
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Related Product Information for anti-Factor XI antibody

Factor XI (F.XI, plasma thromboplastin antecedent) is a coagulation protein produced in the liver that circulates in plasma at approximately 5 ug/ml (30 nM). The mass of F.XI is 160 kDa as determined by SDS-PAGE under non-reducing conditions and 80 kDa upon reduction. F.XI consists of two identical 80 kDa subunits linked by disulphide bonds. Each subunit consists of a tandem repeat of four apple domains followed by a serine protease catalytic domain. Cleavage of F.XI by activated factor XII or thrombin converts each subunit into a two-chain form and generates two active sites per F.XIa molecule. The mass of F.XIa is 160 kDa unreduced, but upon reduction F.XIa migrates as a heavy chain of 50 kDa and a light chain of 30 kDa. The catalytic site of F.XIa resides in the light chain. In plasma, F.XI or F.XIa circulates in non-covalent 1:1 complex with high molecular weight kininogen, which acts as a cofactor in the activation of F.XI by activated factor XII. The activity of F.XIa is regulated by platelets and by several proteinase inhibitors including, in order of decreasing importance, C1-inhibitor, alpha2antiplasmin, alpha1antitrypsin and antithrombin. Heparin has relatively little effect on the rate of inhibition of F.XIa by antithrombin. The only known natural substrate for activated F.XI (F.XIa) is factor IX (Christmas factor) and the only cofactor required for this reaction is ionized calcium1-3.

References

1. Wuillemin WA, Minnema M, Meijers JCM, Roem D, Erenberg AJM, Nuijens JH, ten Cate H, Hack EC; Inactivation of Factor XIa in Human Plasma Assessed by Measuring Factor XIa-Protease Inhibitor Complexes: Major Role for C1-Inhibitor. Blood 85:1517, 1995.
2. DeLa Cadena R, Watchtfogel YT, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 219-240, J.B. Lippincott Co., Philadelphia, 1994.
3. Baglia FA, Seaman FS, Walsh, PN; The Apple 1 and 4 domains of Factor XI Act to Synergistically Promote the Surface-Mediated Activation of Factor XI by Factor XIIa. Blood 85:2078, 1995.

NCBI and Uniprot Product Information

• NCBI GI #: 14626469
• NCBI Official Full Name: factor XI
• Protein Family: Factor XIIa inhibitor