Sheep Factor VIII:C Polyclonal Antibody | anti-FVIII antibody
Sheep anti-human Factor VIII (FVIII:C), Biotinylated Affinity-Purified IgG
Purity
Affinity purified, Biotinylated IgG.Vial containing mL of affinity-purified IgG conjugated to biotin. Total protein is 0.1 mg.
Synonyms
Factor VIII:C; Polyclonal Antibody; Sheep anti-human Factor VIII (FVIII:C); Biotinylated Affinity-Purified IgG; human; anti-FVIII antibody
Host
Sheep
Clonality
Polyclonal
Specificity
Prior to conjugation, this antibody was specific for FVIII as demonstrated by immunoelectrophoresis and ELISA.
Purity/Purification
Affinity purified, Biotinylated IgG.
Vial containing mL of affinity-purified IgG conjugated to biotin. Total protein is 0.1 mg.
Vial containing mL of affinity-purified IgG conjugated to biotin. Total protein is 0.1 mg.
Form/Format
APIgG-biotin conjugate as a clear, colourless liquid.
Concentration
APIgG-biotin concentration is mg/mL, determined by absorbance using an extinction coefficient (E1%280) of 14. (varies by lot)
Application Notes
Suitable as a source of biotinylated antibodies to Factor VIII.
Immunogen
Human FVIII (FVIII:C) purified from concentrate.
Buffer
Phosphate-buffered saline containing 1 mg/mL bovine albumin and 0.1% sodium azide (w/v), pH 7.4.
Preparation and Storage
Store at 2 degree C to 8 degree C.
Related Product Information for anti-FVIII antibody
Factor VIII (formerly referred to as antihemophilic globulin and Factor VIII:C) is a large glycoprotein (320 kDa) that circulates in plasma at approximately 200 ng/mL. Synthesized in the liver, the majority of Factor VIII is cleaved during expression, resulting in a heterogeneous mixture of partially cleaved forms of FVIII ranging in size from 200-280 kDa. The FVIII is stabilized by association with von Willebrand Factor to form a FVIII-vWF complex required for the normal survival of FVIII in vivo (t1/2 of 8-12 hours). FVIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process FVIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Once dissociated from vWF, FVIIIa is susceptible to inactivation by activated Protein C and by non-enzymatic decay. Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome-linked deficiency of FVIII. The severity of the deficiency generally correlates with the severity of the disease. Some Hemophiliacs (~10%) produce a FVIII protein that is partially or totally inactive. The production of neutralizing antibodies to FVIII also occurs in 5-20% of Hemophiliacs 1-3.
References
1. Lollar P, Fay PJ, Fass DN; Factor VIII and Factor VIIIa. Methods in Enzymology, 222, pg 122, 1993.
2. Hoyer, LW, Wyshock EG, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 109-133, J.B. Lippincott Co., Philadelphia, 1994.
3. Pittman DD, Kaufman RJ. Structure-Function Relationships of Factor VIII Elucidated through Recombinant DNA Technology. Thromb. Haemostas. 61:161-165, 1989.
2. Hoyer, LW, Wyshock EG, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 109-133, J.B. Lippincott Co., Philadelphia, 1994.
3. Pittman DD, Kaufman RJ. Structure-Function Relationships of Factor VIII Elucidated through Recombinant DNA Technology. Thromb. Haemostas. 61:161-165, 1989.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
factor VIII
NCBI Official Symbol
FVIII
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Product Notes
The FVIII (Catalog #AAA512055) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. Suitable as a source of biotinylated antibodies to Factor VIII. Researchers should empirically determine the suitability of the FVIII for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Factor VIII:C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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