Western Blot (WB)
(Anti-FABP5 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human FABP5 Polyclonal Antibody | anti-FABP5 antibody
Anti-FABP5 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
ICC/IF: 1:300-1:10,000
IP: 4-8uL/mg of lysate
Western Blot (WB)
(Anti-FABP5 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-FABP5 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))
Immunofluorescence (IF)
(Immunofluorescence staining of FABP5 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FABP5 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of FABP5 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FABP5 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Immunoprecipitation (IP)
(FABP5 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-FABP5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FABP5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
Immunoprecipitation (IP)
(FABP5 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-FABP5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FABP5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus.[provided by RefSeq, Feb 2011]
Uniprot Description
FABP5: High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 8q21.13
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: protein binding; transporter activity; fatty acid binding; lipid binding
Biological Process: epidermis development; phosphatidylcholine biosynthetic process; glucose metabolic process; glucose transport; lipid metabolic process
Research Articles on FABP5
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Product Notes
The FABP5 fabp5 (Catalog #AAA8106527) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FABP5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FABP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 ICC/IF: 1:300-1:10,000 IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the FABP5 fabp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FABP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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