Goat FABP2 Polyclonal Antibody | anti-FABP2 antibody

Goat anti-FABP2 Antibody

Cat. Num. AAA420047

• Gene Names: FABP2; FABPI; I-FABP
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FABP2; Polyclonal Antibody; Goat anti-FABP2 Antibody; Goat Anti-FABP2; Biotinylated Antibody; fatty acid binding protein 2; intestinal; FABPI; I-FABP; MGC133132; Fatty acid-binding protein; intestinal fatty acid binding protein 2; FABP2 antibody; intestinal antibody; FABPI antibody; I-FABP antibody; MGC133132 antibody; intestinal fatty acid binding protein 2 antibody; anti-FABP2 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EGVEAKRIFKKD
• Sequence Length: 132

• Applicable Applications for anti-FABP2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Immunohistochemistry: In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration, 2-3ug/ml.; Western Blot: A very strong approx 16kDa band observed in Human and Mouse Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.1). In transfected HEK293 transiently expressing FABP2 a band of approx.18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 001ug/ml.
• Immunogen: Peptide with sequence C-EGVEAKRIFKKD, from the C Terminus of the protein sequence according to NP_000125.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.001ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(HEK293 overexpressing FABP2 (RC210206) and probed with (mock transfection in first lane), tested by Origene.)

Immunohistochemistry (IHC)

((2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 194097325
• NCBI GeneID: 2169
• NCBI Accession #: NP_000125.2
• NCBI GenBank Nucleotide #: NM_000134.3
• Molecular Weight: 15,207 Da
• NCBI Official Full Name: fatty acid-binding protein, intestinal
• NCBI Official Synonym Full Names: fatty acid binding protein 2
• NCBI Official Symbol: FABP2
• NCBI Official Synonym Symbols: FABPI; I-FABP
• NCBI Protein Information: fatty acid-binding protein, intestinal
• UniProt Protein Name: Fatty acid-binding protein, intestinal
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP2
• UniProt Synonym Gene Names: FABPI; I-FABP
• UniProt Entry Name: FABPI_HUMAN

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP2: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Chromosomal Location of Human Ortholog: 4q28-q31

Cellular Component: cytosol

Molecular Function: fatty acid binding; protein binding; transporter activity

Biological Process: digestion; transport; triacylglycerol catabolic process