F(ab')2 IgG Secondary Antibody | IgG secondary antibody

Rabbit F(ab')2 Anti-Rat IgG(H+L)-BIOT

Cat. Num. AAA679409

• Reactivity: Rat
• Applications: ELISA, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: F(ab')2 IgG; Polyclonal Secondary Antibody; Rabbit F(ab')2 Anti-Rat IgG(H+L)-BIOT; IgG secondary antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit F(ab')2 IgG
• Specificity: Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
• Form/Format: Phosphate buffered saline containing <0.1% sodium azide
• Concentration: 0.5mg/mL (varies by lot)

• Applicable Applications for IgG secondary antibody: ELISA (EIA), Fluorescence-Linked Immunosorbent Assay (FLISA), Immunohistochemistry-Frozen (IHC-F) Sections, Immunohistochemistry-Paraffin (IHC-P) Sections, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Quality tested applications for relevant formats include-; ELISA FLISA; Other referenced applications for relevant formats include-; Immunohistochemistry-Frozen Sections 1; Immunohistochemistry-Paraffin Sections 2; Immunocytochemistry 3-6; Western Blot 7-9
• Source: Pepsin digest of Rabbit Anti-Rat IgG(H+L)
• Conjugation: BIOT (Biotin)
• Cross Adsorption: None; may react with immunoglobulins from other species and the light chains of other rat immunoglobulins
• Preparation and Storage: Store at 2-8 degree C. Please refer to product specific SDS

ELISA (EIA)

(ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Rabbit F(ab')2 Anti-Rat IgG(H+L)-BIOT followed by Streptavidin-HRP .)

References

1. Cariappa A, Shoham T, Liu H, Levy S, Boucheix C, Pillai S. The CD9 tetraspanin is not required for the development of peripheral B cells or for humoral immunity. J Immunol. 2005;175:2925-30. (IHC-FS)
2. Büller NV, Rosekrans SL, Metcalfe C, Heijmans J, van Dop WA, Fessler E, et al. Stromal Indian hedgehog signaling is required for intestinal adenoma formation in mice. Gastroenterology. 2015;148:170-80. (IHC-PS)
3. Poglitsch CL, Thompson NL. Interaction of antibodies with Fc receptors in substrate-supported planar membranes measured by total internal reflection fluorescence microscopy. Biochemistry. 1990;29:248-54. (ICC)
4. Sergejeva S, Johansson A, Malmhäll C, Lötvall J. Allergen exposure-induced differences in CD34+ cell phenotype: relationship to eosinophilopoietic responses in different compartments. Blood. 2004;103:1270-7. (ICC)
5. Johansson A, Sergejeva S, Sjöstrand M, Lee JJ, Lötvall J. Allergen-induced traffic of bone marrow eosinophils, neutrophils and lymphocytes to airways. Eur J Immunol. 2004;34:3135-45. (ICC)
6. Rådinger M, Bossios A, Alm A, Jeurink P, Lu Y, Malmhäll C, et al. Regulation of allergen-induced bone marrow eosinophilopoiesis: role of CD4+ and CD8+ T cells. Allergy. 2007;62:1410-8. (ICC)
7. Bumgarner GW, Zampell JC, Nagarajan S, Poloso NJ, Dorn AS, D'Souza MJ, et al. Modified cell ELISA to determine the solubilization of cell surface proteins: Applications in GPI-anchored protein purification. J Biochem Biophys Methods. 2005;64:99-109. (WB)
8. Ivanov S, Bozinovski S, Bossios A, Valadi H, Vlahos R, Malmhäll C, et al. Functional relevance of the IL-23-IL-17 axis in lungs in vivo. Am J Respir Cell Mol Biol. 2007;36:442-51. (WB)
9. Lässer C, Alikhani VS, Ekström K, Eldh M, Paredes PT, Bossios A, et al. Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages. J Transl Med. 2011;9:9. (WB)