Rabbit anti-Human, Mouse FA96A Polyclonal Antibody | anti-FA96A antibody

FA96A, CT (FAM96A, MIP18 family protein FAM96A) (FITC)

Cat. Num. AAA6297529

• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FA96A; Polyclonal Antibody; CT (FAM96A; MIP18 family protein FAM96A) (FITC); FAM96A; MIP18 family protein FAM96A; anti-FA96A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FA96A antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FA96A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-146 amino acids from the C-terminal region of human FA96A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FA96A Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FA96A antibody detected the FA96A protein (arrow).)

Western Blot (WB)

(FA96A Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the FA96A antibody detected the FA96A protein (arrow).)

Immunohistochemistry (IHC)

(FA96A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FA96A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FA96A Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-FA96A antibody

Antibodies; FAM

References

Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 14149934
• NCBI GeneID: 84191
• NCBI Accession #: NP_115607.1
• NCBI GenBank Nucleotide #: NM_032231.5
• UniProt Accession #: Q9H5X1; A6NKS1; B2R5F8; B7Z8Z5
• Molecular Weight: 11,634 Da
• NCBI Official Full Name: MIP18 family protein FAM96A isoform a
• NCBI Official Synonym Full Names: family with sequence similarity 96 member A
• NCBI Official Symbol: FAM96A
• NCBI Protein Information: MIP18 family protein FAM96A
• UniProt Protein Name: MIP18 family protein FAM96A
• UniProt Gene Name: FAM96A
• UniProt Entry Name: FA96A_HUMAN

Uniprot Description

FAM96A: May play a role in chromosome segregation through establishment of sister chromatid cohesion. Belongs to the MIP18 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: metal ion binding

Biological Process: iron-sulfur cluster assembly; chromosome segregation