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Western Blot (WB) (All lanes : Anti-F2 Antibody (N-Term) at 1:2000 dilutionLane 1: HL-60 whole cell lysatesLane 2: A549 whole cell lysatesLane 3: human plasma lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human F2 Polyclonal Antibody | anti-F2 antibody

F2 Antibody (N-Term)

Gene Names
F2; PT; THPH1; RPRGL2
Reactivity
Human
Applications
Western Blot
Synonyms
F2; Polyclonal Antibody; F2 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Prothrombin; Coagulation factor II; Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain; anti-F2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This F2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-79 amino acids from human F2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
622
Applicable Applications for anti-F2 antibody
Western Blot (WB)
Application Notes
WB ~~ 1:2000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-F2 Antibody (N-Term) at 1:2000 dilutionLane 1: HL-60 whole cell lysatesLane 2: A549 whole cell lysatesLane 3: human plasma lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-F2 Antibody (N-Term) at 1:2000 dilutionLane 1: HL-60 whole cell lysatesLane 2: A549 whole cell lysatesLane 3: human plasma lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-F2 antibody
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Product Categories/Family for anti-F2 antibody
References
Degen S.J.F.,et al.Biochemistry 26:6165-6177(1987). Wang W.,et al.Haemophilia 10:94-97(2004). Ota T.,et al.Nat. Genet. 36:40-45(2004). Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. Degen S.J.F.,et al.Biochemistry 22:2087-2097(1983).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70037
NCBI Official Full Name
prothrombin isoform 1 preproprotein
NCBI Official Synonym Full Names
coagulation factor II (thrombin)
NCBI Official Symbol
F2
NCBI Official Synonym Symbols
PT; THPH1; RPRGL2
NCBI Protein Information
prothrombin
UniProt Protein Name
Prothrombin
Protein Family
UniProt Gene Name
F2
UniProt Entry Name
THRB_HUMAN

NCBI Description

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Research Articles on F2

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Product Notes

The F2 f2 (Catalog #AAA9216778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The F2 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's F2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the F2 f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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