Rabbit anti-Human Ezrin Polyclonal Antibody | anti-EZR antibody

Anti-Ezrin Antibody, Rabbit Polyclonal

Cat. Num. AAA8106614

• Gene Names: EZR; CVL; CVIL; VIL2
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Ezrin; Polyclonal Antibody; Anti-Ezrin Antibody; Rabbit Polyclonal; Ezrin/villin 2 Antibody; Rabbit PAb; Antigen Affinity Purified; ezrin; Anti-CVIL Antibody; Anti-CVL Antibody; Anti-HEL-S-105 Antibody; Anti-VIL2 Antibody; anti-EZR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Ezrin/villin 2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-EZR antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:10,000-1:20,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human Ezrin/villin 2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Ezrin/villin 2 (rh Ezrin/villin 2). Ezrin/villin 2 specific IgG was purified by Human Ezrin/villin 2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human EZR in human brain with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(EZR was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate2 uL anti-EZR rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EZR rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 69 kDaObserved band size: 90 kDa)

Western Blot (WB)

(Anti-EZR rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:69 kDaObserved band size:90 kDa)

Related Product Information for anti-EZR antibody

VIL2, also known as villin-2, belongs to the ERM protein family. It is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. VIL2 contains 1 FERM domain and may be involved in connections of major cytoskel

Product Categories/Family for anti-EZR antibody

B Cell Receptor Signaling Pathway

References

Grnholm M, et al. (1999) Homotypic and heterotypic interaction of the neurofibromatosis 2 tumor suppressor protein merlin and the ERM protein ezrin. J Cell Sci. 112(6):895-904.Sitaraman, et al. (2002) The adenosine 2b receptor is recruited to the plasma membrane and associates with E3KARP and Ezrin upon agonist stimulation. J Biol Chem. 277(36):33188-95.Gould KL, et al. (1989) cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. EMBO J. 8(13):4133-42.

NCBI and Uniprot Product Information

• NCBI GI #: 161702986
• NCBI GeneID: 7430
• NCBI Accession #: NP_001104547.1
• NCBI GenBank Nucleotide #: NM_001111077.1
• UniProt Accession #: P15311; P23714; Q4VX75; Q96CU8; Q9NSJ4; E1P5A8
• Molecular Weight: 69,413 Da
• NCBI Official Full Name: ezrin
• NCBI Official Synonym Full Names: ezrin
• NCBI Official Symbol: EZR
• NCBI Official Synonym Symbols: CVL; CVIL; VIL2
• NCBI Protein Information: ezrin; p81; villin-2; cytovillin 2; villin 2 (ezrin)
• UniProt Protein Name: Ezrin
• Protein Family: Ezrin
• UniProt Gene Name: EZR
• UniProt Synonym Gene Names: VIL2
• UniProt Entry Name: EZRI_HUMAN

NCBI Description

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: cortical cytoskeleton; extracellular space; focal adhesion; microvillus; basolateral plasma membrane; T-tubule; actin filament; cytosol; actin cytoskeleton; lipid raft; ruffle; extrinsic to membrane; membrane; microvillus membrane; apical part of cell; apical plasma membrane; plasma membrane; nucleolus; uropod; vesicle; filopodium

Molecular Function: actin filament binding; protein domain specific binding; protein binding; cell adhesion molecule binding

Biological Process: microvillus biogenesis; axon guidance; actin filament bundle formation; regulation of cell shape; leukocyte adhesion; filopodium formation; cytoskeletal anchoring; receptor internalization; membrane to membrane docking; establishment of epithelial cell polarity