Rabbit anti-Human EWSR1 Polyclonal Antibody | anti-EWSR1 antibody

EWSR1 Polyclonal Antibody

Cat. Num. AAA2565810

• Gene Names: EWSR1; EWS; bK984G1.4
• Reactivity: Human
• Applications: Immunofluorescence
• Purity: Affinity purification
• Synonyms: EWSR1; Polyclonal Antibody; EWSR1 Polyclonal Antibody; EWS; EWS-FLI1; bK984G1.4; anti-EWSR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-EWSR1 antibody: Immunofluorescence (IF)
• Application Notes: IF: 1:50-1:100
• Immunogen: Recombinant fusion protein of human EWSR1 (NP_0011567571)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using EWSR1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-EWSR1 antibody

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t (11;22) (q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.

Product Categories/Family for anti-EWSR1 antibody

Cancer; Epigenetics and Nuclear Signaling; Tags and Cell Markers; Polyclonal Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 253970502
• NCBI GeneID: 2130
• NCBI Accession #: NP_001156757.1
• NCBI GenBank Nucleotide #: NM_001163285.1
• UniProt Accession #: Q01844; Q5THL0; Q92635; Q96FE8; Q96MN4; Q96MX4; Q9BWA2; B0QYK1
• Molecular Weight: 68,478 Da
• NCBI Official Full Name: RNA-binding protein EWS isoform 3
• NCBI Official Synonym Full Names: EWS RNA-binding protein 1
• NCBI Official Symbol: EWSR1
• NCBI Official Synonym Symbols: EWS; bK984G1.4
• NCBI Protein Information: RNA-binding protein EWS; Ewing sarcoma breakpoint region 1; Ewings sarcoma EWS-Fli1 (type 1) oncogene
• UniProt Protein Name: RNA-binding protein EWS
• Protein Family: RNA-binding protein
• UniProt Gene Name: EWSR1
• UniProt Synonym Gene Names: EWS
• UniProt Entry Name: EWS_HUMAN

NCBI Description

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]

Uniprot Description

EWS: an oncogenic RNA-binding protein. Might normally function as a repressor. Binds RPB3, SF1, calmodulin and RNA. Interacts with and regulated by Pyk2. Relocates from cytoplasm to ribosomes upon Pyk2 activation. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. 5 alternatively-spliced isoforms have been described.

Protein type: Oncoprotein; Transcription, coactivator/corepressor; RNA-binding

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nucleus

Molecular Function: calmodulin binding; identical protein binding; protein binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

Disease: Ewing Sarcoma