Typical Testing Data/Standard Curve (for reference only)
Rat D-amino-acid oxidase ELISA Kit | DAO elisa kit
Rat diamine oxidase, DAO ELISA Kit
Reactivity
Rat
Synonyms
D-amino-acid oxidase; Rat diamine oxidase; DAO ELISA Kit; Rat diamine oxidase (DAO) ELISA Kit; DAAO; DAMOX; MGC35381; OXDA; ; DAO elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat DAO. No significant cross-reactivity or interference between Rat DAO and analogues was observed.
Sequence Length
347
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.78 mIU/ml-50 mIU/ml
Sensitivity
0.195 mIU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DAO elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DAO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DAO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,590 Da
NCBI Official Full Name
D-amino-acid oxidase
NCBI Official Symbol
DAO
NCBI Protein Information
D-amino-acid oxidase; DAAO; DAMOX; D-amino acid oxidase
UniProt Protein Name
D-amino-acid oxidase
Protein Family
UniProt Gene Name
DAO
UniProt Synonym Gene Names
DAAO; DAMOX; DAO
UniProt Entry Name
OXDA_RABIT
Uniprot Description
Function: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids
Catalytic activity: A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor: FAD.
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Product Notes
The Rat DAO dao (Catalog #AAA700248) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700248 ELISA Kit recognizes Rat DAO. It is sometimes possible for the material contained within the vial of "D-amino-acid oxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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