Western Blot (WB)
(Anti-MECOM rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:118 kDaObserved band size:170 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human EVI1/MECOM Polyclonal Antibody | anti-MECOM antibody
Anti-EVI1/MECOM Antibody, Rabbit Polyclonal
ICC/IF: 1:300-1:10,000
Western Blot (WB)
(Anti-MECOM rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:118 kDaObserved band size:170 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-MECOM rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:118 kDaObserved band size:170 kDa(We are unsure as to the identity of these extra bands.))
Immunofluorescence (IF)
(Immunofluorescence staining of MECOM in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MECOM polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of MECOM in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MECOM polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Uniprot Description
MDS1: A chromosomal aberration involving MDS1 is found in a form of acute myeloid leukemia (AML). Translocation t(3;21) with AML1. 6 isoforms of the human protein are produced by alternative promoter.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 3q26.2
Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; histone deacetylase complex; nuclear speck; nucleus
Molecular Function: protein binding; protein homodimerization activity; DNA binding; metal ion binding; transcription factor activity
Biological Process: embryonic forelimb morphogenesis; pericardium development; negative regulation of JNK cascade; transcription, DNA-dependent; in utero embryonic development; regulation of cell cycle; apoptosis; positive regulation of transcription, DNA-dependent; neutrophil homeostasis; embryonic hindlimb morphogenesis; regulation of cell proliferation; post-embryonic development; regulation of transcription, DNA-dependent; response to bacterium; forebrain development; negative regulation of programmed cell death; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell differentiation; inflammatory response; negative regulation of transforming growth factor beta receptor signaling pathway
Research Articles on MECOM
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Product Notes
The MECOM mecom (Catalog #AAA8100835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EVI1/MECOM Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EVI1/MECOM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the MECOM mecom for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EVI1/MECOM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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