Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EVI1/AML1 Polyclonal Antibody | anti-MECOM antibody
Goat anti-EVI1/AML1 Antibody
Western Blot: Approx 170kDa band observed in lysates of cell lines A549 and HepG2 (calculated MW of 126kDa according to NP_001098547.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Uniprot Description
MDS1: A chromosomal aberration involving MDS1 is found in a form of acute myeloid leukemia (AML). Translocation t(3;21) with AML1. 6 isoforms of the human protein are produced by alternative promoter.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 3q26.2
Cellular Component: cytoplasm; cytosol; Golgi apparatus; histone deacetylase complex; intracellular membrane-bound organelle; nuclear speck; nucleoplasm; nucleus
Molecular Function: DNA binding; histone-lysine N-methyltransferase activity; metal ion binding; protein binding; protein homodimerization activity; transcription factor activity
Biological Process: apoptosis; cell differentiation; negative regulation of JNK cascade; negative regulation of programmed cell death; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of transcription, DNA-dependent; regulation of cell cycle; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on MECOM
Similar Products
Product Notes
The MECOM mecom (Catalog #AAA422330) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EVI1/AML1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's EVI1/AML1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 170kDa band observed in lysates of cell lines A549 and HepG2 (calculated MW of 126kDa according to NP_001098547.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the MECOM mecom for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DKESLHSTSH. It is sometimes possible for the material contained within the vial of "EVI1/AML1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
