Rabbit anti-Human, Mouse ETV4 Polyclonal Antibody | anti-ETV4 antibody

ETV4 Polyclonal Antibody

Cat. Num. AAA3011344

• Gene Names: ETV4; E1AF; PEA3; E1A-F; PEAS3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ETV4; Polyclonal Antibody; ETV4 Polyclonal Antibody; Adenovirus E1A enhancer binding protein; Adenovirus E1A enhancer-binding protein; E1A F; E1A-F; ETS translocation variant 4; ets variant gene 4 (E1A enhancer binding protein E1AF); ETV4_HUMAN; PEA3; PEA3 protein; PEAS3; Polyomavirus enhancer activator 3; Polyomavirus enhancer activator 3 homolog; Protein PEA3; anti-ETV4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ETV4 polyclonal antibody detects endogenous levels of ETV4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 484

• Applicable Applications for anti-ETV4 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human ETV4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ETV4 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using ETV4 polyclonal antibody)

Related Product Information for anti-ETV4 antibody

Several members of the Ets gene family are known to encode sequence-specific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-a. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.

NCBI and Uniprot Product Information

• NCBI GI #: 118918421
• NCBI GeneID: 2118
• NCBI Accession #: NP_001073143.1
• NCBI GenBank Nucleotide #: NP_001073143.1
• UniProt Accession #: P43268
• Molecular Weight: ~ 54kDa
• NCBI Official Full Name: ETS translocation variant 4 isoform 1
• NCBI Official Synonym Full Names: ETS variant transcription factor 4
• NCBI Official Symbol: ETV4
• NCBI Official Synonym Symbols: E1AF; PEA3; E1A-F; PEAS3
• NCBI Protein Information: ETS translocation variant 4
• UniProt Protein Name: ETS translocation variant 4
• Protein Family: ETS translocation
• UniProt Gene Name: ETV4
• UniProt Synonym Gene Names: E1AF; PEA3; Protein PEA3
• UniProt Entry Name: ETV4_HUMAN

Uniprot Description

ETV4: Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. Belongs to the ETS family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleolus

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; motor axon guidance; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation; cell differentiation; negative regulation of mammary gland epithelial cell proliferation