Rabbit ETK Polyclonal Antibody | anti-ETK antibody

ETK (K35) Polyclonal Antibody

Cat. Num. AAA3001255

• Gene Names: BMX; ETK; PSCTK2; PSCTK3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ETK; Polyclonal Antibody; ETK (K35) Polyclonal Antibody; Cytoplasmic tyrosine-protein kinase BMX; Bone marrow tyrosine kinase gene in chromosome X protein; Epithelial and endothelial tyrosine kinase; NTK38; BMX; anti-ETK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ETK (K35) polyclonal antibody detects endogenous levels of ETK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 675

• Applicable Applications for anti-ETK antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human ETK.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ETK (K35) pAb in paraffin-embeddedhuman liver carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ETK (K35) pAb in paraffin-embeddedhuman rectum carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ETK antibody

ETK, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family. It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways. Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo. In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40.

NCBI and Uniprot Product Information

• NCBI GI #: 4502435
• NCBI GeneID: 660
• NCBI Accession #: NP_001712.1
• NCBI GenBank Nucleotide #: NP_001712.1
• UniProt Accession #: P51813
• Molecular Weight: ~ 78kDa
• NCBI Official Full Name: cytoplasmic tyrosine-protein kinase BMX isoform 1
• NCBI Official Synonym Full Names: BMX non-receptor tyrosine kinase
• NCBI Official Symbol: BMX
• NCBI Official Synonym Symbols: ETK; PSCTK2; PSCTK3
• NCBI Protein Information: cytoplasmic tyrosine-protein kinase BMX
• UniProt Protein Name: Cytoplasmic tyrosine-protein kinase BMX
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BMX
• UniProt Synonym Gene Names: ETK
• UniProt Entry Name: BMX_HUMAN

NCBI Description

This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]

Uniprot Description

Etk: a tyrosine kinase of the Tec family. Activity is required for IL6-induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: extrinsic to internal side of plasma membrane; cytosol

Molecular Function: signal transducer activity; protein binding; protein-tyrosine kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; adaptive immune response; apoptosis; protein amino acid autophosphorylation; innate immune response; mesoderm development; cell adhesion; signal transduction; cell differentiation; T cell receptor signaling pathway; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; cell structure disassembly during apoptosis