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Western Blot (WB)
(Western blot analysis of ETHE1 antibody (Center) in mouse kidney tissue lysates (35ug/lane). ETHE1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse ETHE1 Polyclonal Antibody | anti-ethE1 antibody
ETHE1, ID (Protein ETHE1, Mitochondrial, Ethylmalonic Encephalopathy Protein 1, Hepatoma Subtracted Clone One Protein, HSCO)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
ETHE1; Polyclonal Antibody; ID (Protein ETHE1; Mitochondrial; Ethylmalonic Encephalopathy Protein 1; Hepatoma Subtracted Clone One Protein; HSCO); Anti -ETHE1; anti-ethE1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ETHE1. Species Crossreactivity: mouse.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-ethE1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunogen
Synthetic peptide selected from the Center region of human ETHE1 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ETHE1 antibody (Center) in mouse kidney tissue lysates (35ug/lane). ETHE1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ETHE1 antibody (Center) in mouse kidney tissue lysates (35ug/lane). ETHE1 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293 cells using ETHE1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293 cells using ETHE1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunohistochemistry (IHC)
(ETHE1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ETHE1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ETHE1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ETHE1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of ETHE1 (arrow) using rabbit polyclonal ETHE1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ETHE1 gene.)
Western Blot (WB)
(Western blot analysis of ETHE1 (arrow) using rabbit polyclonal ETHE1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ETHE1 gene.)
Related Product Information for anti-ethE1 antibody
ETHE1 is a sulfur dioxygenase that localizes within the mitochondrial matrix. The enzyme functions in sulfide catabolism. Mutations in its gene result in ethylmalonic encephalopathy.
Product Categories/Family for anti-ethE1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Ethe1
NCBI Official Symbol
ethE1
NCBI Protein Information
ETHE1 protein, mitochondrial precursor; Ethe1
Protein Family
Similar Products
Product Notes
The ethE1 (Catalog #AAA627209) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ETHE1, ID (Protein ETHE1, Mitochondrial, Ethylmalonic Encephalopathy Protein 1, Hepatoma Subtracted Clone One Protein, HSCO) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ETHE1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the ethE1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETHE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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