Western Blot (WB)
(Anti-ETFB rabbit polyclonal antibody at 1:500 dilution Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:27 kDa Observed band size:27 kDa)
Rabbit ETFB Polyclonal Antibody | anti-ETFB antibody
Anti-ETFB Antibody, Rabbit Polyclonal
Reactivity
HumanPredicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
ETFB; Polyclonal Antibody; Anti-ETFB Antibody; Rabbit Polyclonal; ETFB Antibody; Rabbit PAb; Antigen Affinity Purified; electron-transfer-flavoprotein; beta polypeptide; Anti-FP585 Antibody; Anti-MADD Antibody; anti-ETFB antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ETFB
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ETFB antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:500
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human ETFB fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ETFB fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ETFB rabbit polyclonal antibody at 1:500 dilution Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:27 kDa Observed band size:27 kDa)
Western Blot (WB)
(Anti-ETFB rabbit polyclonal antibody at 1:500 dilution Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:27 kDa Observed band size:27 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human ETFB in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ETFB in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
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Product Notes
The ETFB (Catalog #AAA8108696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ETFB Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's ETFB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC-P: 1:200-1:1000 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the ETFB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETFB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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