anti-Human Estrogen Receptor b1, b2 Polyclonal Antibody

Anti - Estrogen Receptor b1, b2 (ERb1, 2)

Cat. Num. AAA617269

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Estrogen Receptor b1; b2; Polyclonal Antibody; Anti - Estrogen Receptor b1; b2 (ERb1; 2); anti-Estrogen Receptor b1; b2 antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The 19-aa human immunogenic peptide shows 78% homology with the mouse ERb1 and 73% with wit teh rat ER b1 (1-3).. The sequence is also conserved in rat ERb2 and the variants of ERb1/b2 (ERb1d3 & ERb2d3). It is not clear, at present, if all of these ERb isoforms can be seen and distinguished by this antibody because of very small size differences in various ERb isoforms. Actual crossreactivity of antibodies in all species is not established.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-Estrogen Receptor b1, b2 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. The predicted size of the ERb receptor is approx. 54-kD (1).; ELISA: ~1:100,000 using 50-100ng control peptide/well.; Optimal dilutions to be determined by the researcher.
• Immunogen: A 19 AA C-terminal peptide from the human ERb was synthesized, coupled to KLH
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Estrogen Receptor b1, b2 antibody

Mouse/rat ERb1 gene encodes a protein of 485aa with a calculated size of approx. 54kD (1). Rat ERb1 is highly conserved in the DNA-binding domain (>90% homology) and the C-terminal ligand binding domain (55% homology). The A/B domain, the hinge region and the F-domain are not conserved. Rat ERb1 is primarily expressed in prostate, ovary, lung, bladder, brain, uterus, and testis. Most recently functional variants of ERb1, termed ERb2, have been cloned. ERbb has an additional 18 aa as a results of in-frame mutation within the ligand-binding domain. ERb1 is expressed in ovary, prostate, pituitary, brain, and muscle. Variants of both ERb1 and ERb2 (ERb1d3, ERb2d3) were detected that show deletion of 39 aa in the DNA-binding domain. Both ERb1 and ERb2 specifically bind to ER response element. ERa, b1 and ERb2 have been found to heterodimerize with each other.

Product Categories/Family for anti-Estrogen Receptor b1, b2 antibody

Abs to Receptors

References

• 1. Kuiper GG et al (1996) Proc Natl Acad Sci 93:5925-5930.
• 2. Tremblay, GB et al (1997) Mol. Endocrinol. 11:353-365.
• 3. Mosselman S et al (1996) FEBS Lett. 392:49-53.
• 4. Peterson DN et al (198) Endocrinology 139:1082.
• 5. Maruyama K et al (1998) BBRC 246:142.