Rabbit anti-Human Estrogen Receptor alpha, Polyclonal Antibody

Estrogen Receptor alpha, (Ser167) (ER, ER alpha, Era, ESR, ESR, ESR1, ESRA, Estradiol Receptor, Estrogen Receptor, Estrogen Receptor 1)

Cat. Num. AAA6007384

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Estrogen Receptor alpha; ; Polyclonal Antibody; (Ser167) (ER; ER alpha; Era; ESR; ESR1; ESRA; Estradiol Receptor; Estrogen Receptor; Estrogen Receptor 1); Anti -Estrogen Receptor alpha; anti-Estrogen Receptor alpha; antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ESR1. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Estrogen Receptor alpha, antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500-1:1000; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic non-phosphopeptide corresponding to aa surrounding Ser167 of human ESR1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Breast (formalin-fixed, paraffin-embedded) stained with ESR1 antibody at 10ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-Estrogen Receptor alpha, antibody

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha. Particularly, phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity.

Product Categories/Family for anti-Estrogen Receptor alpha, antibody

Antibodies; Abs to Receptors; Estrogen

NCBI and Uniprot Product Information

• NCBI GI #: 5821726
• NCBI Official Full Name: estrogen receptor alpha

Uniprot Description

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.