Rabbit anti-Human ESRRB Polyclonal Antibody | anti-ESRRB antibody

ESRRB, ID (ESRRB, ERRB2, ESRL2, NR3B2, Steroid hormone receptor ERR2, ERR beta-2, Estrogen receptor-like 2, Estrogen-related receptor beta, Nuclear receptor subfamily 3 group B member 2) (APC)

Cat. Num. AAA6296867

• Gene Names: ESRRB; ERR2; ERRb; ESRL2; NR3B2; DFNB35
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ESRRB; Polyclonal Antibody; ID (ESRRB; ERRB2; ESRL2; NR3B2; Steroid hormone receptor ERR2; ERR beta-2; Estrogen receptor-like 2; Estrogen-related receptor beta; Nuclear receptor subfamily 3 group B member 2) (APC); Nuclear receptor subfamily 3 group B member 2; anti-ESRRB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ESRRB
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ESRRB antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ESRRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283~312 amino acids from the Center region of human ESRRB.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ESRRB Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ESRRB (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ESRRB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ESRRB Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ESRRB antibody

ESRRB encodes a protein with similarity to the estrogen receptor. Its function is unknown; however, a similar protein in mouse plays an essential role in placental development.

Product Categories/Family for anti-ESRRB antibody

Antibodies; Receptors; Estrogen

References

Xu, J., et.al., Proc. Natl. Acad. Sci. U.S.A. 107 (5), 2136-2140 (2010). Bombail, V., et.al., Mol. Cell. Endocrinol. 314 (1), 53-61 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 238550159
• NCBI GeneID: 2103
• NCBI Accession #: NP_004443
• NCBI GenBank Nucleotide #: NM_004452.3
• UniProt Accession #: O95718; Q5F0P7; Q5F0P8; Q9HCB4; A2VDJ2; B6ZGU4
• Molecular Weight: 48,054 Da
• NCBI Official Full Name: steroid hormone receptor ERR2
• NCBI Official Synonym Full Names: estrogen-related receptor beta
• NCBI Official Symbol: ESRRB
• NCBI Official Synonym Symbols: ERR2; ERRb; ESRL2; NR3B2; DFNB35
• NCBI Protein Information: steroid hormone receptor ERR2; ERR-beta; ERRbeta-2; ERR beta-2; nuclear receptor ERRB2; orphan nuclear receptor; estrogen receptor-like 2; estrogen-related nuclear receptor beta; nuclear receptor subfamily 3 group B member 2
• UniProt Protein Name: Steroid hormone receptor ERR2
• Protein Family: Steroid hormone receptor
• UniProt Gene Name: ESRRB
• UniProt Synonym Gene Names: ERRB2; ESRL2; NR3B2; ERR-beta
• UniProt Entry Name: ERR2_HUMAN

NCBI Description

This gene encodes a protein with similarity to the estrogen receptor. Its function is unknown; however, a similar protein in mouse plays an essential role in placental development. [provided by RefSeq, Jul 2008]

Uniprot Description

ERR2: Nuclear receptor, may regulate ESR1 transcriptional activity. Defects in ESRRB are the cause of deafness autosomal recessive type 35 (DFNB35). DFNB35 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the nuclear hormone receptor family. NR3 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor activity; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; steroid hormone receptor activity; transcription factor binding; steroid binding

Biological Process: transcription initiation from RNA polymerase II promoter; embryonic placenta development; trophectodermal cell proliferation; intracellular receptor-mediated signaling pathway; regulation of transcription, DNA-dependent; stem cell maintenance; trophectodermal cellular morphogenesis; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling

Disease: Deafness, Autosomal Recessive 35