Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Esophageal Cancer Related Gene 4 Polyclonal Antibody | anti-ECRG4 antibody
Esophageal Cancer Related Gene 4 (ECRG4) Polyclonal Antibody
Gene Names
C2orf40; ECRG4
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Esophageal Cancer Related Gene 4; Polyclonal Antibody; Esophageal Cancer Related Gene 4 (ECRG4) Polyclonal Antibody; C2orf40; Augurin; anti-ECRG4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ECRG4. It has been selected for its ability to recognize ECRG4 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
0.67mg/ml (varies by lot)
Sequence Length
148
Applicable Applications for anti-ECRG4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
ECRG4 (Asn32~Tyr148)
Quality Control
Content: The quality control contains recombinant ECRG4 (Asn32~Tyr148) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2084086
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded heart tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded heart tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Thyroid cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Thyroid cancer Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,183 Da
NCBI Official Full Name
augurin
NCBI Official Synonym Full Names
chromosome 2 open reading frame 40
NCBI Official Symbol
C2orf40
NCBI Official Synonym Symbols
ECRG4
NCBI Protein Information
augurin
UniProt Protein Name
Augurin
UniProt Gene Name
C2orf40
UniProt Synonym Gene Names
ECRG4
Uniprot Description
C2orf40: Probable hormone that may induce senescence of oligodendrocyte and neural precursor cells, characterized by G1 arrest, RB1 dephosphorylation and accelerated CCND1 and CCND3 proteasomal degradation. Belongs to the augurin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2q12.2
Cellular Component: extracellular space; transport vesicle
Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
Research Articles on ECRG4
Similar Products
Product Notes
The ECRG4 c2orf40 (Catalog #AAA2032603) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Esophageal Cancer Related Gene 4 (ECRG4) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Esophageal Cancer Related Gene 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ECRG4 c2orf40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Esophageal Cancer Related Gene 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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