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Goat Escherichia coli, O, K Serotypes Polyclonal Antibody

Escherichia coli, O, K Serotypes (E. coli) (FITC)

Applications
Immunofluorescence
Purity
Highly Purified
Purified IgG fraction to a purity of > 95%.
Synonyms
Escherichia coli; O; K Serotypes; Polyclonal Antibody; K Serotypes (E. coli) (FITC); Anti -Escherichia coli; anti-Escherichia coli; K Serotypes antibody
Ordering
For Research Use Only!
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
Many "O" and "K" antigenic serotypes of Escherichia coli. Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae.
Purity/Purification
Highly Purified
Purified IgG fraction to a purity of > 95%.
Form/Format
Supplied as a liquid in PBS, pH 7.2, BSA (10mg/ml), 0.1% sodium azide. Conjugated to FITC.
Applicable Applications for anti-Escherichia coli, O, K Serotypes antibody
Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence.
Dilution: Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Immunogen
Mixture of E. coli serotypes
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Escherichia coli, O, K Serotypes antibody
Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls.[1][2] The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2,[3] or by preventing the establishment of pathogenic bacteria within the intestine.[4][5] E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination.[6][7] The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885,[6] and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.[8]

A strain of E. coli is a sub-group within the species that has unique characteristics that distinguish it from other E. coli strains. These differences are often detectable only on the molecular level; however, they may result in changes to the physiology or lifecycle of the bacterium. For example, a strain may gain pathogenic capacity, the ability to use a unique carbon source, the ability to inhabit a particular ecological niche or the ability to resist antimicrobial agents. Different strains of E. coli are often host-specific, making it possible to determine the source of fecal contamination in environmental samples.[6][7] For example, knowing which E. coli strains are present in a water sample allows to make assumptions about whether the contamination originated from a human, another mammal or a bird.
Product Categories/Family for anti-Escherichia coli, O, K Serotypes antibody

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Product Notes

The Escherichia coli, O, K Serotypes (Catalog #AAA627510) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Escherichia coli, O, K Serotypes can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). Suitable for use in Immunofluorescence. Dilution: Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Researchers should empirically determine the suitability of the Escherichia coli, O, K Serotypes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Escherichia coli, O, K Serotypes, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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