Immunofluorescence (IF)
(MBS9600358 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse ES8L2 Polyclonal Antibody | anti-ES8L2 antibody
ES8L2 Antibody
Gene Names
EPS8L2; EPS8R2; DFNB106
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ES8L2; Polyclonal Antibody; ES8L2 Antibody; Epidermal growth factor receptor kinase substrate 8 like protein 2; Epidermal growth factor receptor kinase substrate 8-like protein 2; Epidermal growth factor receptor pathway substrate 8 like protein 2; Epidermal growth factor receptor pathway substrate 8 related protein 2; Epidermal growth factor receptor pathway substrate 8-related protein 2; EPS8 like 2; EPS8 like protein 2; EPS8 related protein 2; EPS8-like protein 2; EPS8-related protein 2; EPS8L 2; EPS8L2; EPS8R 2; EPS8R2; ES8L2_HUMAN; FLJ16738; FLJ21935; FLJ22171; MGC126530; MGC3088; anti-ES8L2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ES8L2 antibody detects endogenous levels of ES8L2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
715
Applicable Applications for anti-ES8L2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human ES8L2
Subcellular Location
Cytoplasm.
Tissue Specificity
Detected in fibroblasts and placenta.
Predicted Cross Reactivity
Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus
Similarity
Zebrafish (100%), Bovine (100%), Horse (100%), Dog (100%), Chicken (91%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9600358 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9600358 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis on HeLa cell lysate using ES8L2 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on HeLa cell lysate using ES8L2 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-ES8L2 antibody
Description: EPS8L2 Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. Belongs to the EPS8 family. Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin. 2 isoforms of the human protein are produced by alternative splicing.
Function: Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. In the cochlea, is required for stereocilia maintenance in adult hair cells (By similarity).
Subunit Structure: Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin.
Similarity: Belongs to the EPS8 family.
Function: Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. In the cochlea, is required for stereocilia maintenance in adult hair cells (By similarity).
Subunit Structure: Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin.
Similarity: Belongs to the EPS8 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 81 kDa
Predicted: 81 kDa
Predicted: 81 kDa
NCBI Official Full Name
epidermal growth factor receptor kinase substrate 8-like protein 2
NCBI Official Synonym Full Names
EPS8 like 2
NCBI Official Symbol
EPS8L2
NCBI Official Synonym Symbols
EPS8R2; DFNB106
NCBI Protein Information
epidermal growth factor receptor kinase substrate 8-like protein 2
UniProt Protein Name
Epidermal growth factor receptor kinase substrate 8-like protein 2
UniProt Gene Name
EPS8L2
UniProt Synonym Gene Names
EPS8R2; EPS8-like protein 2; EPS8-related protein 2
NCBI Description
This gene encodes a member of the EPS8 gene family. The encoded protein, like other members of the family, is thought to link growth factor stimulation to actin organization, generating functional redundancy in the pathways that regulate actin cytoskeletal remodeling. [provided by RefSeq, Dec 2008]
Uniprot Description
Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. In the cochlea, is required for stereocilia maintenance in adult hair cells ().
Research Articles on ES8L2
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Product Notes
The ES8L2 eps8l2 (Catalog #AAA9600358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ES8L2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ES8L2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ES8L2 eps8l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ES8L2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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