Rabbit anti-Human Erythropoietin (EPO) Polyclonal Antibody | anti-EPO antibody

Polyclonal Antibody to Erythropoietin (EPO)

Cat. Num. AAA2090525

• Gene Names: EPO; EP; MVCD2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Erythropoietin (EPO); Polyclonal Antibody; Polyclonal Antibody to Erythropoietin (EPO); EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin; anti-EPO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against EPO. It has been selected for its ability to recognize EPO in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 193

• Applicable Applications for anti-EPO antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western Blot: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL
• Immunogen: Eukaryotic Erythropoietin (EPO)
• Cross Reactivity: Human
• Buffer Formulation: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant EPO, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Brain Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 62240997
• NCBI GeneID: 2056
• NCBI Accession #: NP_000790.2
• NCBI GenBank Nucleotide #: NM_000799.3
• UniProt Accession #: P01588; Q2M2L6; Q549U2; Q9UDZ0; Q9UEZ5; Q9UHA0
• Molecular Weight: 21,307 Da
• NCBI Official Full Name: erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: EPO
• NCBI Official Synonym Symbols: EP; MVCD2
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: EPO

NCBI Description

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: erythropoietin receptor binding; hormone activity; protein binding; protein kinase activator activity

Biological Process: activation of protein kinase activity; acute-phase response; aging; apoptosis; blood circulation; cell proliferation; embryo implantation; erythrocyte differentiation; erythrocyte maturation; hemoglobin biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; peptidyl-serine phosphorylation; positive regulation of activated T cell proliferation; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of Ras protein signal transduction; positive regulation of transcription, DNA-templated; positive regulation of tyrosine phosphorylation of STAT protein; response to axon injury; response to electrical stimulus; response to estrogen; response to hyperoxia; response to lipopolysaccharide; response to salt stress; response to testosterone stimulus; response to vitamin A; signal transduction

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2