Western Blot (WB)
(Western blot analysis of ERp72 in human colon tissue lysate with ERp72 antibody at (A) 1 and (B) 2 μg/ml.)
Rabbit ERp72 Polyclonal Antibody | anti-PDIA4 antibody
ERp72 Antibody
Gene Names
PDIA4; ERP70; ERP72; ERp-72
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity chromatography purified via peptide column.
Synonyms
ERp72; Polyclonal Antibody; ERp72 Antibody; Protein disulfide isomerase family A member 4; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ERp70; ERp-72; anti-PDIA4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ERP72 antibody is human, mouse and rat reactive.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
645
Applicable Applications for anti-PDIA4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
ERp72 antibody can be used for detection of ERp72 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Immunogen
ERp72 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ERp72.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Human Colon Tissue Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of ERp72 in human colon tissue lysate with ERp72 antibody at (A) 1 and (B) 2 μg/ml.)
Western Blot (WB)
(Western blot analysis of ERp72 in human colon tissue lysate with ERp72 antibody at (A) 1 and (B) 2 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ERp72 in rat colon tissue with ERp72 antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ERp72 in rat colon tissue with ERp72 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ERp72 in rat colon tissue with ERp72 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ERp72 in rat colon tissue with ERp72 antibody at 20 μg/mL.)
Related Product Information for anti-PDIA4 antibody
Protein disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (1,2). ER stress protein 72 (ERp72), also designated protein disulfide-isomerase A4, is involved in the catalysis of protein -S-S- bond rearrangement (3). It plays a role in the formation and isomerization of disulfide bonds (3,4).
Product Categories/Family for anti-PDIA4 antibody
References
Huppa JB and Ploegh HL. The eS-Sence of -SH in the ER. Cell 1998; 92:145-8. Ellgaard L and Ruddock LW. The human protein disulphide isomerase family: substrate interactions and functional properties. EMBO Rep. 2005; 6:28-32. Mazzarella RA, Srinivasan M, Haugejorden SM, et al. ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase. J. Biol. Chem. 1990; 265:1094-101. Satoh M, Shimada A, Keino H, et al. Functional characterization of 3 thioredoxin homology domains of ERp72. Cell Stress Chaperones 2005; 10:278-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 71 kDa
Observed: 75 kDa
Observed: 75 kDa
NCBI Official Full Name
protein disulfide-isomerase A4
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 4
NCBI Official Symbol
PDIA4
NCBI Official Synonym Symbols
ERP70; ERP72; ERp-72
NCBI Protein Information
protein disulfide-isomerase A4
UniProt Protein Name
Protein disulfide-isomerase A4
Protein Family
UniProt Gene Name
PDIA4
UniProt Synonym Gene Names
ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72
UniProt Entry Name
PDIA4_HUMAN
Uniprot Description
PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.
Protein type: Endoplasmic reticulum; Isomerase; EC 5.3.4.1; Chaperone
Chromosomal Location of Human Ortholog: 7q35
Cellular Component: cell surface; endoplasmic reticulum lumen; melanosome
Molecular Function: protein binding; protein disulfide isomerase activity
Biological Process: cell redox homeostasis; metabolic process; protein folding; protein secretion
Research Articles on PDIA4
Similar Products
Product Notes
The PDIA4 pdia4 (Catalog #AAA154373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERp72 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERp72 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). ERp72 antibody can be used for detection of ERp72 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the PDIA4 pdia4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERp72, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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