Western Blot (WB)
(Western blot analysis of ERp57 expression in HepG2 cell lysate.)
Rabbit anti-Human ERp57 Polyclonal Antibody | anti-ERp57 antibody
Anti-ERp57 Rabbit pAb
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
ERp57; Polyclonal Antibody; Anti-ERp57 Rabbit pAb; ERp60; ERp61; Glucose Regulated Protein 58 Kd; HsT17083; p58; PDIA3; Phospholipase C alpha; PI PLC; anti-ERp57 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ERp57 Antibody detects endogenous levels of total ERp57
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
505
Applicable Applications for anti-ERp57 antibody
Western Blot (WB), Immunhistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50
IHC: 1:50-1:200
IP: 1:50
Immunogen
A synthesized peptide derived from human ERp57
Subcellular Location
Melanosome
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of ERp57 expression in HepG2 cell lysate.)
Western Blot (WB)
(Western blot analysis of ERp57 expression in HepG2 cell lysate.)
Related Product Information for anti-ERp57 antibody
Detected in the flagellum and head region of spermatozoa (at protein level).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57kDa
NCBI Official Full Name
protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 3
NCBI Official Symbol
PDIA3
NCBI Official Synonym Symbols
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
NCBI Protein Information
protein disulfide-isomerase A3
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Gene Name
PDIA3
UniProt Synonym Gene Names
ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
NCBI Description
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]
Uniprot Description
CautionWas originally thought to be a phosphatidylinositol 4,5-bisphosphate phosphodiesterase type I (phospholipase C-alpha).
Research Articles on ERp57
Similar Products
Product Notes
The ERp57 pdia3 (Catalog #AAA4750853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ERp57 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERp57 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunhistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:50. Researchers should empirically determine the suitability of the ERp57 pdia3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERp57, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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