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Western Blot (WB) (Western blot analysis of ERp57 (Grp58): Lane 1: MW marker, Lane 2: CHO-K1 lysate, Lane 3: Human Liver Microsomes, Lane 4: Rat Liver Microsomes, Lane 5: Mouse Liver Microsomes.)

Rabbit ERp57 Polyclonal Antibody | anti-PDIA3; ERp60; ERp61; GRP57; Grp58 antibody

ERp57 polyclonal antibody

Gene Names
Pdia3; ER60; ERp57; Grp58
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit
Applications
Immunohistochemistry, Western Blot
Purity
Protein A affinity purified.
Synonyms
ERp57; Polyclonal Antibody; ERp57 polyclonal antibody; anti-PDIA3; ERp60; ERp61; GRP57; Grp58 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit
Clonality
Polyclonal
Specificity
Recognizes human, mouse, rat, bovine, dog, guinea pig, hamster, monkey, pig and rabbit ERp57. Detects a band of ~57kDa by Western blot.
Purity/Purification
Protein A affinity purified.
Form/Format
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Sequence Length
505
Applicable Applications for anti-PDIA3; ERp60; ERp61; GRP57; Grp58 antibody
Immunohistochemistry (IHC) (PS), Western Blot (WB)
Application Notes
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~57kDa by Western blot.
Immunogen
Native rat liver ERp57.
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of ERp57 (Grp58): Lane 1: MW marker, Lane 2: CHO-K1 lysate, Lane 3: Human Liver Microsomes, Lane 4: Rat Liver Microsomes, Lane 5: Mouse Liver Microsomes.)

Western Blot (WB) (Western blot analysis of ERp57 (Grp58): Lane 1: MW marker, Lane 2: CHO-K1 lysate, Lane 3: Human Liver Microsomes, Lane 4: Rat Liver Microsomes, Lane 5: Mouse Liver Microsomes.)
Related Product Information for anti-PDIA3; ERp60; ERp61; GRP57; Grp58 antibody
ERp57 (also known as Grp58), a member of the Thioredoxin superfamily, is an ER protein with disulfide isomerase activity. It has been shown to increase after oncogenic transformation and to be a component of the MHC Class I peptide-loading complex.
Product Categories/Family for anti-PDIA3; ERp60; ERp61; GRP57; Grp58 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,623 Da
NCBI Official Full Name
Protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A, member 3
NCBI Official Symbol
Pdia3
NCBI Official Synonym Symbols
ER60; ERp57; Grp58
NCBI Protein Information
protein disulfide-isomerase A3
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Gene Name
Pdia3
UniProt Synonym Gene Names
Erp60; Grp58; p58; ER protein 57; ERp57; ER protein 60; ERp60
UniProt Entry Name
PDIA3_RAT

NCBI Description

interacts with thiazide-sensitive sodium-chloride cotransporter in the kidney and is induced by glucose deprivation [RGD, Feb 2006]

Uniprot Description

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: Isomerase; Protease; Phospholipase; Apoptosis; EC 5.3.4.1; Endoplasmic reticulum; Chaperone

Cellular Component: acrosome; apical plasma membrane; cell surface; cytoplasm; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region; extracellular space; focal adhesion; melanosome; mitochondrial intermembrane space; mitochondrion; myelin sheath; nucleus; plasma membrane; smooth endoplasmic reticulum; TAP complex

Molecular Function: MHC class I protein binding; peptidase activity; protein binding; protein disulfide isomerase activity; protein-disulfide reductase (glutathione) activity

Biological Process: cell redox homeostasis; circadian rhythm; protein folding; proteolysis; response to ethanol; response to genistein; response to hyperoxia; response to hypoxia; response to nutrient levels

Research Articles on PDIA3; ERp60; ERp61; GRP57; Grp58

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Product Notes

The PDIA3; ERp60; ERp61; GRP57; Grp58 pdia3 (Catalog #AAA565344) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERp57 polyclonal antibody reacts with Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's ERp57 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (PS), Western Blot (WB). Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~57kDa by Western blot. Researchers should empirically determine the suitability of the PDIA3; ERp60; ERp61; GRP57; Grp58 pdia3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERp57, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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