Rabbit anti-Human ERP29 Polyclonal Antibody | anti-ERP29 antibody

ERP29, ID (Endoplasmic Reticulum Resident Protein 29, Endoplasmic Reticulum Resident Protein 31, Endoplasmic Reticulum Resident Protein 28, ERp31, ERp28, C12orf8)

Cat. Num. AAA626505

• Gene Names: ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: ERP29; Polyclonal Antibody; ID (Endoplasmic Reticulum Resident Protein 29; Endoplasmic Reticulum Resident Protein 31; Endoplasmic Reticulum Resident Protein 28; ERp31; ERp28; C12orf8); Anti -ERP29; anti-ERP29 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ERP29.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ERP29 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the Center region of human ERP29 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis using MBS626505 and cell line lysates (35ug/lane) Lane 1: A2058 Lane 2: A375 Lane 3: MCF7 Lane 4: NCI-H460 Lane 5: Y79 The ERP29 protein(arrow) was detected .)

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS626505 either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2))

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue using MBS626505 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS626505 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H292 cells using MBS626505 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ERP29 antibody

ERP29 shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.

Product Categories/Family for anti-ERP29 antibody

Antibodies; Abs to Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5803013
• NCBI GeneID: 10961
• NCBI Accession #: NP_006808.1
• NCBI GenBank Nucleotide #: NM_006817.3
• UniProt Accession #: P30040; Q3MJC3; Q6FHT4
• Molecular Weight: 28,993 Da
• NCBI Official Full Name: endoplasmic reticulum resident protein 29 isoform 1
• NCBI Official Synonym Full Names: endoplasmic reticulum protein 29
• NCBI Official Symbol: ERP29
• NCBI Official Synonym Symbols: ERp28; ERp31; PDIA9; PDI-DB; C12orf8
• NCBI Protein Information: endoplasmic reticulum resident protein 29; endoplasmic reticulum resident protein 28; endoplasmic reticulum resident protein 31; endoplasmic reticulum lumenal protein ERp28; protein disulfide isomerase family A, member 9
• UniProt Protein Name: Endoplasmic reticulum resident protein 29
• Protein Family: Endoplasmic reticulum resident protein
• UniProt Gene Name: ERP29
• UniProt Synonym Gene Names: C12orf8; ERP28
• UniProt Entry Name: ERP29_HUMAN

NCBI Description

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

ERP29: Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: transport vesicle; cell surface; smooth endoplasmic reticulum; membrane; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome

Molecular Function: protein binding; protein homodimerization activity; chaperone binding; protein disulfide isomerase activity

Biological Process: intracellular protein transport; negative regulation of protein secretion; protein folding; activation of MAPK activity; protein unfolding; protein secretion; positive regulation of protein amino acid phosphorylation