Rabbit ERN2 Polyclonal Antibody | anti-ERN2 antibody

ERN2 Polyclonal Antibody

Cat. Num. AAA2524709

• Gene Names: ERN2; IRE1b; IRE1-BETA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: ERN2; Polyclonal Antibody; ERN2 Polyclonal Antibody; IRE1b; IRE1-BETA; anti-ERN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 2.4mg/mL (varies by lot)
• Sequence Length: 926

• Applicable Applications for anti-ERN2 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide of human ERN2
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer tissue using ERN2 Polyclonal Antibody at dilution 1:40)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ERN2 Polyclonal Antibody at dilution 1:40)

Related Product Information for anti-ERN2 antibody

The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1alpha and IRE1beta are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes.

Product Categories/Family for anti-ERN2 antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 148342546
• NCBI GeneID: 10595
• Molecular Weight: 102,480 Da
• NCBI Official Full Name: ERN2 protein
• NCBI Official Synonym Full Names: endoplasmic reticulum to nucleus signaling 2
• NCBI Official Symbol: ERN2
• NCBI Official Synonym Symbols: IRE1b; IRE1-BETA
• NCBI Protein Information: serine/threonine-protein kinase/endoribonuclease IRE2; ER to nucleus signalling 2; ERN2; IRE1 beta; IRE1, S. cerevisiae, homolog of; endoplasmic reticulum to nucleus signalling 2; endoplasmic reticulum-to-nucleus signaling 2; hIRE2p; inositol-requiring 1 beta; inositol-requiring protein 2
• UniProt Protein Name: Serine/threonine-protein kinase/endoribonuclease IRE2
• Protein Family: Serine/threonine-protein kinase/endoribonuclease
• UniProt Gene Name: ERN2
• UniProt Synonym Gene Names: hIRE2p; IRE1b
• UniProt Entry Name: ERN2_HUMAN

Uniprot Description

IRE2: Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: Apoptosis; Membrane protein, integral; Protein kinase, Other; Ribonuclease; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; IRE family

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: protein serine/threonine kinase activity; endonuclease activity; magnesium ion binding; ATP binding; ribonuclease activity

Biological Process: transcription, DNA-dependent; rRNA catabolic process; apoptotic chromosome condensation; cell cycle arrest; mRNA processing; negative regulation of transcription, DNA-dependent; protein amino acid phosphorylation