M-CSF active protein

Human M-CSF Protein

Cat. Num. AAA9718704

• Gene Names: CSF1; MCSF; CSF-1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: M-CSF; Human M-CSF Protein; Recombinant Human M-CSF Protein (Macrophage colony-stimulating factor 1) expressed in Human Cells; CSF-1; MCSF; Macrophage colony-stimulating factor 1; CSF1; M-CSF active protein

• Host: Human Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4.
• Sequence: Amino acid sequence derived from human CSF1 (NP_757349.1) (Met1-Asn190) was expressed with an initial Met.
• Sequence Length: 438

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug protein as determined by the LAL method.
• Protein Length: The recombinant human CSF1 consists 158 amino acids and predicts a molecular mass of 18.4 kDa.
• Activity: Measured in a cell proliferation assay using M?NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.6-6.3 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human M-CSF protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human M-CSF protein.)

Related Product Information for M-CSF active protein

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte/ macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

NCBI and Uniprot Product Information

• NCBI GI #: 27262663
• NCBI GeneID: 1435
• NCBI Accession #: NP_757349.1
• NCBI GenBank Nucleotide #: NP_757349.1
• UniProt Accession #: P09603
• Molecular Weight: 18.4 kDa
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform b
• NCBI Official Synonym Full Names: colony stimulating factor 1
• NCBI Official Symbol: CSF1
• NCBI Official Synonym Symbols: MCSF; CSF-1
• NCBI Protein Information: macrophage colony-stimulating factor 1

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]