Western Blot (WB)
(Western Blot analysis of ERK5 on Hela cells lysates using anti-ERK5 antibody at 1/1,000 dilution.)
Rabbit ERK5 Polyclonal Antibody | anti-ERK5 antibody
ERK5 Polyclonal Antibody
Gene Names
MAPK7; BMK1; ERK4; ERK5; PRKM7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
ERK5; Polyclonal Antibody; ERK5 Polyclonal Antibody; MAP kinase 7; MAPK 7; BMK-1; ERK-5; MAPK7; Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK1; BMK1 Kinase; EC 2.7.11.24; ERK 4; ERK 5; ERK4; Extracellular signal regulated kinase 5; Extracellular signal-regulated kinase 5; Mitogen activated protein kinase 7; Mitogen-activated protein kinase 7; MK07; OTTHUMP00000065906; OTTHUMP00000065907; PRKM 7; PRKM7; PROTEIN KINASE; MITOGEN-ACTIVATED; 7; anti-ERK5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ERK5 polyclonal antibody detects endogenous levels of ERK5 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
816
Applicable Applications for anti-ERK5 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of ERK5 on Hela cells lysates using anti-ERK5 antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of ERK5 on Hela cells lysates using anti-ERK5 antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining ERK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining ERK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-ERK5 antibody
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
115kDa
NCBI Official Full Name
mitogen-activated protein kinase 7 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 7
NCBI Official Symbol
MAPK7
NCBI Official Synonym Symbols
BMK1; ERK4; ERK5; PRKM7
NCBI Protein Information
mitogen-activated protein kinase 7
UniProt Protein Name
Mitogen-activated protein kinase 7
UniProt Gene Name
MAPK7
UniProt Synonym Gene Names
BMK1; ERK5; PRKM7; MAP kinase 7; MAPK 7; BMK-1; ERK-5
UniProt Entry Name
MK07_HUMAN
NCBI Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Research Articles on ERK5
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Product Notes
The ERK5 mapk7 (Catalog #AAA3012658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERK5 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERK5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1,000-1:2,000 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ERK5 mapk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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