Western Blot (WB)
((0.3ug/ml) staining of 293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ERK1/MAPK3 Polyclonal Antibody | anti-MAPK3 antibody
Goat anti-ERK1/MAPK3 Antibody
Western Blot: Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of 293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of 293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing ERK1 (RC204196) and probed (mock transfection in second lane), tested by Origene)
Western Blot (WB)
(HEK293 overexpressing ERK1 (RC204196) and probed (mock transfection in second lane), tested by Origene)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.
Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: caveola; cytoskeleton; cytosol; early endosome; focal adhesion; Golgi apparatus; late endosome; microtubule cytoskeleton; mitochondrion; nuclear envelope; nucleoplasm; nucleus; protein complex; pseudopodium
Molecular Function: ATP binding; kinase activity; MAP kinase activity; phosphatase binding; phosphotyrosine binding; protein binding; protein serine/threonine kinase activity
Biological Process: activation of MAPK activity; apoptosis; arachidonic acid metabolic process; axon guidance; Bergmann glial cell differentiation; BMP signaling pathway; cartilage development; cell cycle; DNA damage induced protein phosphorylation; fibroblast growth factor receptor signaling pathway; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; nuclear translocation of MAPK; outer ear morphogenesis; peptidyl-serine phosphorylation; phosphorylation; platelet activation; positive regulation of cyclase activity; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; positive regulation of transcription from RNA polymerase II promoter; positive regulation of translation; protein amino acid phosphorylation; protein complex assembly; regulation of cytoskeleton organization and biogenesis; regulation of phosphoinositide 3-kinase cascade; regulation of stress-activated MAPK cascade; regulation of transcription factor activity; response to exogenous dsRNA; response to toxin; sensory perception of pain; thymus development; thyroid gland development; transcription initiation from RNA polymerase I promoter; viral reproduction
Research Articles on MAPK3
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Product Notes
The MAPK3 mapk3 (Catalog #AAA421060) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ERK1/MAPK3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1/MAPK3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the MAPK3 mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GGEPRRTEGV GP. It is sometimes possible for the material contained within the vial of "ERK1/MAPK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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