Rabbit ERK1 Polyclonal Antibody | anti-MAPK3 antibody

Rabbit anti ERK1 (pThr202)

Cat. Num. AAA225242

• Gene Names: MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• Reactivity: Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: ERK1; Polyclonal Antibody; Rabbit anti ERK1 (pThr202); ERK1 (pThr202) Antibody; anti-MAPK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 379

• Applicable Applications for anti-MAPK3 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-Paraffin: 1:50-1:100; WB: 1:500-1:2000
• Immunogen: Phospho specific-peptide corresponding to residues surrounding threonine 202 of human ERK1
• Preperation: Antiserum to ERK1 (pThr202) was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of extracts from HEK-293 cells untreated or treated with PMA using Rabbit anti ERK1 (pThr202) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with PMA (200 nM) for 30 minutes)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ERK1 (pThr202) antibody)

Related Product Information for anti-MAPK3 antibody

Rabbit anti ERK1 (pThr202) antibody recognizes ERK1, also known as MAPK3, when phosphorylated at threonine 202. ERK1 is a member of the Mitogen Activated Protein Kinase (MAPK) family. MAPKs constitute a diverse set of signaling molecules. In the case of ERK1, a cascade is initiated by binding activation of a membrane bound receptor which causes activation of a small GTPase, RAS, which in turn activates the protein kinase activity of the RAF protein. RAF in turn phosphorylates and activates MEK. MEK induces phosphorylation of ERK1 at residues threonine 202 and tyrosine 204. Phosphorylation of the residues corresponds to activation of ERK1 and mediation of its diverse biological functions including cell growth, differentiation, motility, and adhesion via its effects on transcription, translation, cytoskeletal rearrangement and cross talk with other signaling pathways

NCBI and Uniprot Product Information

• NCBI GI #: 232066
• NCBI GeneID: 5595
• UniProt Accession #: P27361; Q8NHX1; A8CZ58; B0LPG3
• Molecular Weight: 40,088 Da
• NCBI Official Full Name: Mitogen-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 3
• NCBI Official Symbol: MAPK3
• NCBI Official Synonym Symbols: ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: MAPK3
• UniProt Synonym Gene Names: ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.