Western Blot (WB)
(Fig. Western Blot analysis of various cells using ERK 8 Polyclonal Antibody.)
Rabbit anti-Human, Mouse ERK 8 Polyclonal Antibody | anti-ERK8 antibody
ERK 8 Polyclonal Antibody
Gene Names
MAPK15; ERK7; ERK8
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ERK 8; Polyclonal Antibody; ERK 8 Polyclonal Antibody; Rabbit Anti-ERK 8 Polyclonal Antibody; MAPK15; ERK7; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8; anti-ERK8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ERK 8 Polyclonal Antibody detects endogenous levels of ERK 8 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
544
Applicable Applications for anti-ERK8 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:10000
IHC-P: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:10000
Immunogen
Synthesized peptide derived from human ERK 8 around the non-phosphorylation site of T175/Y177
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig. Western Blot analysis of various cells using ERK 8 Polyclonal Antibody.)
Western Blot (WB)
(Fig. Western Blot analysis of various cells using ERK 8 Polyclonal Antibody.)
Related Product Information for anti-ERK8 antibody
MAPK15 (mitogen-activated protein kinase 15, ERK8) is a 544 amino acid protein that belongs to the CMGC Ser/Thr protein kinase family (MAP kinase subfamily). MAP kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. MAPK15 functions as a catalytic kinase using ATP to produce ADP and a phosphoprotein. A TXY motif, containing one threonine and one tyrosine residue, activates the MAP kinases upon phosphorylation. MAPK15 is a ubiquitously expressed protein with highest expression found in lung and kidney.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitogen-activated protein kinase 15
NCBI Official Synonym Full Names
mitogen-activated protein kinase 15
NCBI Official Symbol
MAPK15
NCBI Official Synonym Symbols
ERK7; ERK8
NCBI Protein Information
mitogen-activated protein kinase 15
UniProt Protein Name
Mitogen-activated protein kinase 15
UniProt Gene Name
MAPK15
UniProt Synonym Gene Names
ERK7; ERK8; MAP kinase 15; MAPK 15; ERK-7; ERK-8
UniProt Entry Name
MK15_HUMAN
Uniprot Description
ERK7: In vitro, phosphorylates MBP. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; Erk7 subfamily
Chromosomal Location of Human Ortholog: -
Cellular Component: extracellular region; intracellular; nucleus
Molecular Function: MAP kinase activity; SH3 domain binding; ATP binding
Biological Process: negative regulation of DNA replication; positive regulation of protein ubiquitination; protein amino acid autophosphorylation; positive regulation of protein catabolic process; MAPKKK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; response to estradiol stimulus
Research Articles on ERK8
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Product Notes
The ERK8 mapk15 (Catalog #AAA9702009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERK 8 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ERK 8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC-P: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the ERK8 mapk15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK 8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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