Rabbit anti-Human ERCC6L Polyclonal Antibody | anti-ERCC6L antibody

ERCC6L Antibody

Cat. Num. AAA9630728

• Gene Names: ERCC6L; PICH; RAD26L
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ERCC6L; Polyclonal Antibody; ERCC6L Antibody; ATP dependent helicase ERCC6 like; ATP-dependent helicase ERCC6-like; DNA excision repair protein ERCC 6 like; DNA excision repair protein ERCC-6-like; ERC6L_HUMAN; ERCC 6L; Ercc6l; Excision repair cross complementing rodent repair deficiency complementation group 6 like; Excision repair protein ERCC6 like; FLJ20105; MGC131695; PICH; Plk1 interacting checkpoint helicase; PLK1-interacting checkpoint helicase; SNF2/RAD54 family protein; Tumor antigen BJ HCC 15; Tumor antigen BJ-HCC-15; anti-ERCC6L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ERCC6L Antibody detects endogenous levels of ERCC6L.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ERCC6L antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ERCC6L.
• Conjugation: Unconjugated
• Similarity: Belongs to the SNF2/RAD54 helicase family.
• Post Translational Modifications: Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
• Subunit Structure: Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization. Interacts (via N-terminal TPR repeat) with BEND3 (via BEN domains 1 and 3); the interaction is direct.
• Function: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Functions as ATP-dependent DNA translocase. Can promote Holliday junction branch migration (in vitro).
• Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ERCC6L antibody

Chromosome; Centromere; Kinetochore; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 121948339
• NCBI GeneID: 54821
• UniProt Accession #: Q2NKX8; Q8NCI1; Q96H93; Q9NXQ8
• Molecular Weight: 1250
• NCBI Official Full Name: DNA excision repair protein ERCC-6-like
• NCBI Official Synonym Full Names: excision repair cross-complementation group 6-like
• NCBI Official Symbol: ERCC6L
• NCBI Official Synonym Symbols: PICH; RAD26L
• NCBI Protein Information: DNA excision repair protein ERCC-6-like; tumor antigen BJ-HCC-15; SNF2/RAD54 family protein; ATP-dependent helicase ERCC6-like; excision repair protein ERCC6-like; Plk1-interacting checkpoint helicase; excision repair cross-complementing rodent repair def
• UniProt Protein Name: DNA excision repair protein ERCC-6-like
• Protein Family: DNA excision repair protein
• UniProt Gene Name: ERCC6L
• UniProt Synonym Gene Names: PICH
• UniProt Entry Name: ERC6L_HUMAN

Uniprot Description

PICH: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization. Belongs to the SNF2/RAD54 helicase family.

Protein type: Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; centrosome; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; DNA binding; helicase activity; ATP binding

Biological Process: mitosis; metabolic process; cell division; mitotic cell cycle