Western Blot (WB)
(Anti-CD2BP2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human CD2BP2 Polyclonal Antibody | anti-CD2BP2 antibody
Anti-CD2BP2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CD2BP2; Polyclonal Antibody; Anti-CD2BP2 Antibody; Rabbit Polyclonal; CD2BP2 Antibody; Rabbit PAb; Antigen Affinity Purified; CD2 (cytoplasmic tail) binding protein 2; Anti-FWP010 Antibody; Anti-LIN1 Antibody; Anti-PPP1R59 Antibody; Anti-Snu40 Antibody; Anti-U5-52K Antibody; anti-CD2BP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CD2BP2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CD2BP2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human CD2BP2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CD2BP2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CD2BP2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-CD2BP2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(CD2BP2 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-CD2BP2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD2BP2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size :55 kDa)
Immunoprecipitation (IP)
(CD2BP2 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-CD2BP2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD2BP2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa Observed band size :55 kDa)
Related Product Information for anti-CD2BP2 antibody
The adaptor protein CD2BP2, originally identified as a binding partner of the adhesion molecule CD2, is a pre-spliceosomal assembly factor that utilizes its glycine-tyrosine-phenylalanine (GYF) domain to co-localize with spliceosomal proteins. CD2BP2 confers binding to proline-rich sequences (PRS) via its GYF domain. CD2BP2 plays a crucial role in CD2-triggered T cell activation and nuclear splicing. CD2BP2 is a non-redundant splicing factor that is essential for embryonic development and podocyte integrity.
References
Kofler M, et al. (2004) Recognition sequences for the gyf domain reveal a possible spliceosomal function of cd2bp2. J Biol Chem 279 (27): 28292-28297.Heinze M, et al. (2007) Investigating the functional role of cd2bp2 in t cells. Int Immunol 19 (11): 1313-1318.Albert GI, et al. (2015) The gyf domain protein cd2bp2 is critical for embryogenesis and podocyte function. J Mol Cell Biol 7 (5): 402-414.
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Product Notes
The CD2BP2 (Catalog #AAA8108319) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD2BP2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD2BP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the CD2BP2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD2BP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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