Rabbit anti-Mouse ERBB2 Polyclonal Antibody | anti-ERBB2 antibody

ERBB2, Phosphorylated (Y1197) (Erbb2, Kiaa3023, Neu, Receptor tyrosine-protein kinase erbB-2, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, p185erbB2, CD340) (PE)

Cat. Num. AAA6296370

• Gene Names: Erbb2; Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; l11Jus8; mKIAA3023
• Reactivity: Mouse
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERBB2; Polyclonal Antibody; Phosphorylated (Y1197) (Erbb2; Kiaa3023; Neu; Receptor tyrosine-protein kinase erbB-2; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; p185erbB2; CD340) (PE); Erbb2; CD340; anti-ERBB2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ERBB2 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-mouse ERBB2-Y1197 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1197 of mouse ERBB2.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-mouse ERBB2-Y1197 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-ERBB2 antibody

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Product Categories/Family for anti-ERBB2 antibody

Antibodies; Protein Kinases

References

Cabodi, S., et al. FASEB J. 24(10):3796-3808(2010). Johnson, E., et al. J. Biol. Chem. 285(38):29491-29501(2010). Huck, L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15559-15564(2010). Chuang, T.D., et al. J. Biol. Chem. 285(31):23598-23606(2010). Simeone, L., et al. J. Neurosci. 30(19):6620-6634(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 54873610
• NCBI GeneID: 13866
• NCBI Accession #: NP_001003817.1
• NCBI GenBank Nucleotide #: NM_001003817.1
• UniProt Accession #: P70424; Q61525; Q6ZPE0
• Molecular Weight: 138,579 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 2
• NCBI Official Symbol: Erbb2
• NCBI Official Synonym Symbols: Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; l11Jus8; mKIAA3023
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: Erbb2
• UniProt Synonym Gene Names: Kiaa3023; Neu

Uniprot Description

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization ().