Western Blot (WB)
(All lanes : Anti-Eras Antibody (C-term) at 1:2000 dilutionLane 1: mouse heart lysatesLane 2: rat heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse, rat Eras Polyclonal Antibody | anti-Eras antibody
(Mouse) Eras Antibody (C-term)
Gene Names
Eras; HRAS2; HRasp; ecat5; Ha-Ras2
Reactivity
Mouse, rat
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Eras; Polyclonal Antibody; (Mouse) Eras Antibody (C-term); GTPase ERas; E-Ras; Embryonic stem cell-expressed Ras; anti-Eras antibody
Host
Rabbit
Reactivity
Mouse, rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Eras antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 202-236 amino acids from the C-terminal region of Mouse Eras.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
227
Applicable Applications for anti-Eras antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2000
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-Eras Antibody (C-term) at 1:2000 dilutionLane 1: mouse heart lysatesLane 2: rat heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Eras Antibody (C-term) at 1:2000 dilutionLane 1: mouse heart lysatesLane 2: rat heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Eras Antibody (C-term)at 1:2000 dilution + mouse kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Eras Antibody (C-term)at 1:2000 dilution + mouse kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Eras antibody
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
Product Categories/Family for anti-Eras antibody
References
Takahashi K.,et al.Nature 423:541-545(2003).
Carninci P.,et al.Science 309:1559-1563(2005).
Carninci P.,et al.Science 309:1559-1563(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24321
NCBI Official Full Name
GTPase ERas
NCBI Official Synonym Full Names
ES cell-expressed Ras
NCBI Official Symbol
Eras
NCBI Official Synonym Symbols
HRAS2; HRasp; ecat5; Ha-Ras2
NCBI Protein Information
GTPase ERas
UniProt Protein Name
GTPase ERas
Protein Family
UniProt Gene Name
Eras
UniProt Synonym Gene Names
E-Ras
UniProt Entry Name
RASE_MOUSE
Uniprot Description
ERas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells. Belongs to the small GTPase superfamily. Ras family.
Protein type: G protein, monomeric; G protein, monomeric, Ras; G protein
Cellular Component: membrane; plasma membrane
Molecular Function: GTP binding; nucleotide binding
Biological Process: small GTPase mediated signal transduction; signal transduction
Research Articles on Eras
Similar Products
Product Notes
The Eras eras (Catalog #AAA9202295) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Eras Antibody (C-term) reacts with Mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Eras can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the Eras eras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eras, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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