Rabbit EPS8L2 Polyclonal Antibody | anti-EPS8L2 antibody

EPS8L2 (K30) Polyclonal Antibody

Cat. Num. AAA3002505

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EPS8L2; Polyclonal Antibody; EPS8L2 (K30) Polyclonal Antibody; Epidermal growth factor receptor kinase substrate 8-like protein 2; EPS8-like protein 2; Epidermal growth factor receptor pathway substrate 8-related protein 2; EPS8-related protein 2; EPS8R2; PP13181; anti-EPS8L2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EPS8L2 polyclonal antibody detects endogenous levels of EPS8L2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 715

• Applicable Applications for anti-EPS8L2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 05-60 of Human EPS8L2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of EPS8L2 (K30) polyclonal antibody at 1:500 dilutionLane1:HCT116 whole cell lysate (10ug) Lane2:A375 whole cell lysate (20ug) Lane3:LOVO whole cell lysate (40ug) Lane4:The Embryo tissue lysate of Mouse (20ug) Lane5:PC12 whole cell lysate (40ug))

Related Product Information for anti-EPS8L2 antibody

Eps8L2 (epidermal growth factor receptor kinase substrate 8-like protein 2), also known as EPS8R2 or PP13181, is a 715 amino acid protein that localizes to the cytoplasm and belongs to the Eps8 (epidermal growth factor receptor pathway substrate 8) family. Expressed in placenta and fibroblasts, Eps8L2 functions to stimulate the guanine exchange activity of Sos 1 (son of sevenless homolog 1), a protein that promotes the exchange of Ras-bound GDP for GTP. Additionally, Eps8L2 is thought to associate with Actin and, via this association, may play a role in membrane ruffling and remodeling of the Actin cytoskeleton. Through its ability to regulate protein activation and cytoskeleton dynamics, Eps8L2 may participate in cell growth and differentiation events within the cell. Eps8L2, a protein that is expressed as two isoforms due to alternative splicing, contains one PID (phosphotyrosine interaction) domain and one SH3 domain.

NCBI and Uniprot Product Information

• NCBI GI #: 21264616
• NCBI Accession #: NP_073609.2
• NCBI GenBank Nucleotide #: NP_073609.2
• UniProt Accession #: Q9H6S3
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: epidermal growth factor receptor kinase substrate 8-like protein 2
• UniProt Protein Name: Epidermal growth factor receptor kinase substrate 8-like protein 2
• Protein Family: Epidermal growth factor receptor kinase substrate 8-like protein
• UniProt Gene Name: EPS8L2
• UniProt Synonym Gene Names: EPS8R2; EPS8-like protein 2
• UniProt Entry Name: ES8L2_HUMAN

Uniprot Description

EPS8L2: Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. Belongs to the EPS8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho; Actin-binding

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: protein complex; cytoplasm; plasma membrane; vesicle

Molecular Function: Rho guanyl-nucleotide exchange factor activity; Rac guanyl-nucleotide exchange factor activity; actin binding

Biological Process: regulation of Rho protein signal transduction; Rho protein signal transduction