Mouse MAGE-1 Monoclonal Antibody | anti-MAGEA1 antibody

MAGE-1 (Target for Cancer Immunotherapy) Mouse Monoclonal Antibody

Cat. Num. AAA438253

• Gene Names: MAGEA1; CT1.1; MAGE1
• Reactivity: Human, Rat, Dog
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: MAGE-1; Monoclonal Antibody; MAGE-1 (Target for Cancer Immunotherapy) Mouse Monoclonal Antibody; MZ2 E; cancer/testis antigen 1.1; CT1.1; MAGE1A; MAGEA1; Melanoma antigen family A 1; Melanoma associated antigen 1; Melanoma associated antigen MZ2 E; anti-MAGEA1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Dog
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM282
• Specificity: Recognizes a protein of 42-46kDa, identified as MAGE-1. This MAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 309

• Applicable Applications for anti-MAGEA1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Human MAGE-A1 full length recombinant protein
• Hu-Chromosome Location: Xq28
• Positive Control: Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Testis stained with MAGE-1 Monoclonal Antibody (SPM282).)

References

Qian, X et al. 2008. Mol Oncol. 2: 81-93

NCBI and Uniprot Product Information

• NCBI GI #: 148276977
• NCBI GeneID: 4100
• NCBI Accession #: NP_004979.3
• NCBI GenBank Nucleotide #: NM_004988.4
• UniProt Accession #: P43355; O00346; B2RC81; AAB33086
• Molecular Weight: 42-46kDa
• NCBI Official Full Name: melanoma-associated antigen 1
• NCBI Official Synonym Full Names: MAGE family member A1
• NCBI Official Symbol: MAGEA1
• NCBI Official Synonym Symbols: CT1.1; MAGE1
• NCBI Protein Information: melanoma-associated antigen 1
• UniProt Protein Name: Melanoma-associated antigen 1
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEA1
• UniProt Synonym Gene Names: MAGE1; MAGE1A; CT1.1
• UniProt Entry Name: MAGA1_HUMAN

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGE-A1: a tumor antigen expressed in a variety of tumor cells and in the testis. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; nucleus; plasma membrane

Molecular Function: histone deacetylase binding; protein binding

Biological Process: negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter