Rabbit anti-Rat Epithelial Sodium Channel, alpha Polyclonal Antibody | anti-Scnn1a antibody

Epithelial Sodium Channel, alpha (ENAC Alpha)

Cat. Num. AAA616250

• Gene Names: Scnn1a; Scnn1a
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Serum; Neat serum.
• Synonyms: Epithelial Sodium Channel; alpha; Polyclonal Antibody; alpha (ENAC Alpha); Anti -Epithelial Sodium Channel; anti-Scnn1a antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Epithelial Sodium Channel, alpha (ENAC Alpha). Species sequence homology: mouse-90%,; bovine-76%; human-75%; rabbit-70% ENACa. No significant homology is detected with ENAC beta, gamma or delta subunits.
• Purity/Purification: Serum; Neat serum.
• Form/Format: Supplied as a liquid with 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-Scnn1a antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:5000; ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies.
• Immunogen: A 20aa peptide sequence near the N-terminus of rat ENACa.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Scnn1a antibody

Tissue acidosis (decrease in pH below the physiological level) that occurs in ischemia, tissue damage or inflammation is accompanied by pain. At the molecular level, H+-gated cation channels are activated by low pH in nociceptive neurons. H+-gated cation channels, members of the NaC/DEG superfamily of Na channels that include amiloride-sensitive epithelial Na+ channel proteins (alpha, beta, gamma and delta-ENAC subunits), are expressed in epithelia of the vertebrate kidney, colon, lung, tongue and brain. The ENAC subunits may form heterotrimeric active Na channel. ENACs are involved in Na and water readsorption, and salty taste transduction) of vertebrate colon, lung, kidney and tongue. The superfamily of DEG/NAC proteins are characterized by intracellular N and C-termini, two TM domains, and a large extracellular loop.

Product Categories/Family for anti-Scnn1a antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 4100904
• NCBI GeneID: 25122
• UniProt Accession #: Q9Z2W4; P37089; Q6IRJ1
• Molecular Weight: 4,255 Da
• NCBI Official Full Name: epithelial sodium channel alpha subunit
• NCBI Official Synonym Full Names: sodium channel, nonvoltage-gated 1 alpha
• NCBI Official Symbol: Scnn1a
• NCBI Official Synonym Symbols: Scnn1a
• NCBI Protein Information: amiloride-sensitive sodium channel subunit alpha; SCNEA; alpha-ENaC; alpha-NaCH; epithelial Na(+) channel subunit alpha; epithelial sodium channel alpha subunit; nonvoltage-gated sodium channel 1 subunit alpha; sodium channel, nonvoltage-gated, type I, alpha
• UniProt Protein Name: Epithelial sodium channel alpha subunit
• Protein Family: Amiloride-sensitive sodium channel
• UniProt Gene Name: Scnn1a
• UniProt Entry Name: Q9Z2W4_RAT

NCBI Description

acts as an epithelial sodium ion channel; regulates salt and fluid transport in the kidney [RGD]

Uniprot Description

ENaC-alpha: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. By aldosterone. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. Activated by WNK1, WNK2, WNK3 and WNK4. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel; Channel, sodium

Cellular Component: cortical actin cytoskeleton; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; cytosol; external side of plasma membrane

Molecular Function: amiloride-sensitive sodium channel activity; WW domain binding; actin binding; sodium channel activity

Biological Process: regulation of body fluid levels; sensory perception of taste; regulation of blood pressure; sodium ion transport; sodium ion homeostasis; multicellular organismal water homeostasis