Rabbit anti-Human EphB6 Polyclonal Antibody | anti-EphB6 antibody

EphB6, NT (S45) (Ephrin Type-A Receptor 6, Tyrosine-protein Kinase Receptor EPH-6, HEP) (MaxLight 405)

Cat. Num. AAA6474470

• Gene Names: EPHB6; HEP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: EphB6; Polyclonal Antibody; NT (S45) (Ephrin Type-A Receptor 6; Tyrosine-protein Kinase Receptor EPH-6; HEP) (MaxLight 405); anti-EphB6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphB6.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-EphB6 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 45-74 in the N-terminal region of human EphB6. Species sequence homology: mouse, rat
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS626952 in A549 cell lysate. EphB6 (arrow) was detected usingMBS626952. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS626952, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS626952 for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-EphB6 antibody

Antibodies; Tyrosine Kinases

References

1. Freywald, A., et al., J. Biol. Chem. 278(12):10150-10156 (2003). 2. Luo, H., et al., J. Clin. Invest. 110(8):1141-1150 (2002). 3. Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). 4. Luo, H., et al., J. Immunol. 167(3):1362-1370 (2001). 5. Tang, X.X., et al., Proc. Natl. Acad. Sci. USA 97(20):10936-10941 (2000). 6.Jun Yu, et al. Clin. Cancer Res., Apr 2010; 16: 2275-2283. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 615276335
• NCBI GeneID: 2051
• NCBI Accession #: NP_004436
• NCBI GenBank Nucleotide #: NM_004445
• UniProt Accession #: O15197; Q2TB23; Q2TB24; A4D2I7; A8CDT5; D3DXD3
• Molecular Weight: 57,498 Da
• NCBI Official Full Name: ephrin type-B receptor 6 isoform a
• NCBI Official Synonym Full Names: EPH receptor B6
• NCBI Official Symbol: EPHB6
• NCBI Official Synonym Symbols: HEP
• NCBI Protein Information: ephrin type-B receptor 6; human kinase-defective Eph-family receptor protein; tyrosine-protein kinase-defective receptor EPH-6
• UniProt Protein Name: Ephrin type-B receptor 6
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB6
• UniProt Entry Name: EPHB6_HUMAN

Uniprot Description

EphB6: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q33-q35

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; cytosol

Molecular Function: ephrin receptor activity; receptor activity; ATP binding

Biological Process: axon guidance; ephrin receptor signaling pathway; protein amino acid phosphorylation