Rabbit anti-Human EPHB4 Polyclonal Antibody | anti-EPHB4 antibody

EPHB4 (Ephrin type-B Receptor 4, Tyrosine-protein Kinase Receptor HTK, Tyrosine-protein Kinase TYRO11, HTK, TYRO11) (MaxLight 490)

Cat. Num. AAA6474460

• Gene Names: EPHB4; HTK; MYK1; TYRO11
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: EPHB4; Polyclonal Antibody; EPHB4 (Ephrin type-B Receptor 4; Tyrosine-protein Kinase Receptor HTK; Tyrosine-protein Kinase TYRO11; HTK; TYRO11) (MaxLight 490); anti-EPHB4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphB4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-EPHB4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Human recombinant EphB4 protein.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EphB4 (arrow) using rabbit polyclonal EphB4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB4 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the EphB4 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-EPHB4 antibody

Antibodies; Receptors; Tyrosine Kinase

References

1. Steinle, J.J., et al., J. Biol. Chem. 277(46):43830-43835 (2002). 2. Suenobu, S., et al., Biochem. Biophys. Res. Commun. 293(3):1124-1131 (2002). 3. Wang, Z., et al., Blood 99(8):2740-2747 (2002). 4. Wilson, M.D., et al., Nucleic Acids Res. 29(6):1352-1365 (2001). 5. Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 32528301
• NCBI GeneID: 2050
• NCBI Accession #: NP_004435
• NCBI GenBank Nucleotide #: NM_004444
• UniProt Accession #: P54760; Q7Z635; Q9BTA5; Q9BXP0; B5A970; B5A971; B5A972
• Molecular Weight: 45,195 Da
• NCBI Official Full Name: ephrin type-B receptor 4
• NCBI Official Synonym Full Names: EPH receptor B4
• NCBI Official Symbol: EPHB4
• NCBI Official Synonym Symbols: HTK; MYK1; TYRO11
• NCBI Protein Information: ephrin type-B receptor 4; ephrin receptor EphB4; hepatoma transmembrane kinase; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK
• UniProt Protein Name: Ephrin type-B receptor 4
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB4
• UniProt Synonym Gene Names: HTK; MYK1; TYRO11
• UniProt Entry Name: EPHB4_HUMAN

Uniprot Description

EphB4: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; cytosol

Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: heart morphogenesis; axon guidance; peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; protein amino acid autophosphorylation; ephrin receptor signaling pathway; angiogenesis; cell adhesion