Rabbit anti-Human, Mouse EphB3 Polyclonal Antibody | anti-EphB3 antibody

EphB3 (EPHB3, ETK2, HEK2, TYRO6, Ephrin type-B receptor 3, EPH-like tyrosine kinase 2, Embryonic kinase 2, Tyrosine-protein kinase TYRO6) (MaxLight 650)

Cat. Num. AAA6296049

• Gene Names: EPHB3; EK2; ETK2; HEK2; TYRO6
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: EphB3; Polyclonal Antibody; EphB3 (EPHB3; ETK2; HEK2; TYRO6; Ephrin type-B receptor 3; EPH-like tyrosine kinase 2; Embryonic kinase 2; Tyrosine-protein kinase TYRO6) (MaxLight 650); EPHB3; Tyrosine-protein kinase TYRO6; anti-EphB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphB3. Species Crossreactivity: mouse
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-EphB3 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: EphB3 antibody is generated from rabbits immunized with EphB3 his fusion protein.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EphB3 Antibody in C6 cell line lysates (35ug/lane).EphB3 (arrow) was detected using the purified Pab)

Western Blot (WB)

(Western blot analysis of EphB3 Antibody in mouse stomach tissue lysates (35ug/lane).EphB3 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(EphB3 Antibody flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EphB3 antibody

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB3, a member of the Tyr family of protein kinases, is a receptor for members of the ephrin-B family; it binds to both ephrin-B1 and-B2. Expression of this Type I membrane protein is ubiquitous. The protein contains putative domains for 2 fibronectin type III and 1 sterile alpha motif (SAM).

Product Categories/Family for anti-EphB3 antibody

Antibodies; Protein Kinases

References

Chiu, S.T., et.al., Carcinogenesis 30 (9), 1475-1486 (2009). Kang, J.U., et.al., BMC Cancer 9, 237 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 17975768
• NCBI GeneID: 2049
• NCBI Accession #: NP_004434
• NCBI GenBank Nucleotide #: NM_004443
• UniProt Accession #: P54753
• Molecular Weight: 107kDa
• NCBI Official Full Name: ephrin type-B receptor 3
• NCBI Official Synonym Full Names: EPH receptor B3
• NCBI Official Symbol: EPHB3
• NCBI Official Synonym Symbols: EK2; ETK2; HEK2; TYRO6
• NCBI Protein Information: ephrin type-B receptor 3
• UniProt Protein Name: Ephrin type-B receptor 3
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB3
• UniProt Synonym Gene Names: ETK2; HEK2; TYRO6; EPH-like kinase 2; EK2; hEK2
• UniProt Entry Name: EPHB3_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. [provided by RefSeq, Mar 2010]

Uniprot Description

EphB3: a ubiquitous receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members: ephrin-B1 and -B2. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.EphB3 contains 1 sterile alpha motif (SAM) domain and 2 fibronectin type III domains.

Protein type: EC 2.7.10.1; Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: integral to plasma membrane; dendrite; plasma membrane; extracellular region; cytosol

Molecular Function: ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; cell migration; peptidyl-tyrosine phosphorylation; thymus development; protein amino acid autophosphorylation; axonal fasciculation; central nervous system projection neuron axonogenesis; digestive tract morphogenesis; palate development; regulation of axonogenesis; corpus callosum development; positive regulation of synaptogenesis; regulation of cell-cell adhesion; ephrin receptor signaling pathway; angiogenesis; urogenital system development; retinal ganglion cell axon guidance