Rabbit anti-Human, Mouse EPHB3 Polyclonal Antibody | anti-EPHB3 antibody

EPHB3 Antibody

Cat. Num. AAA9629044

• Gene Names: EPHB3; ETK2; HEK2; TYRO6
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: EPHB3; Polyclonal Antibody; EPHB3 Antibody; Cek10; EK2; Embryonic kinase 2; EPH Like Tyrosine Kinase 2; EPH receptor B3; EPH-like kinase 2; ephb3; EPHB3_HUMAN; Ephrin receptor EphB3; Ephrin type B receptor 3; Ephrin type-B receptor 3; ETK2; hEK2; Human Embryo Kinase 2; Mdk5; Sek4; TYRO6; Tyrosine protein kinase receptor HEK2; Tyrosine protein kinase TYRO6; Tyrosine-protein kinase TYRO6; anti-EPHB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: EPHB3 Antibody detects endogenous levels of total EPHB3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-EPHB3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
• Post Translational Modifications: Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-614 is required for interaction with SH2 domain-containing proteins.
• Subunit Structure: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).
• Tissue Specificity: Ubiquitous.
• Function: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-EPHB3 antibody

Cell membrane; Single-pass type I membrane protein; Cell projection; Dendrite; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 76803655
• NCBI GeneID: 2049
• UniProt Accession #: P54753; Q7Z740
• Molecular Weight: 110,330 Da
• NCBI Official Full Name: Ephrin type-B receptor 3
• NCBI Official Synonym Full Names: EPH receptor B3
• NCBI Official Symbol: EPHB3
• NCBI Official Synonym Symbols: ETK2; HEK2; TYRO6
• NCBI Protein Information: ephrin type-B receptor 3; EK2; EPH-like kinase 2; embryonic kinase 2; human embryo kinase 2; EPH-like tyrosine kinase 2; EPH-like tyrosine kinase-2; tyrosine-protein kinase TYRO6
• UniProt Protein Name: Ephrin type-B receptor 3
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB3
• UniProt Synonym Gene Names: ETK2; HEK2; TYRO6; EPH-like kinase 2; EK2; hEK2
• UniProt Entry Name: EPHB3_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. [provided by RefSeq, Mar 2010]

Uniprot Description

EphB3: a ubiquitous receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members: ephrin-B1 and -B2. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.EphB3 contains 1 sterile alpha motif (SAM) domain and 2 fibronectin type III domains.

Protein type: EC 2.7.10.1; Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: integral to plasma membrane; dendrite; plasma membrane; extracellular region; cytosol

Molecular Function: ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; cell migration; peptidyl-tyrosine phosphorylation; thymus development; protein amino acid autophosphorylation; axonal fasciculation; central nervous system projection neuron axonogenesis; digestive tract morphogenesis; palate development; regulation of axonogenesis; corpus callosum development; positive regulation of synaptogenesis; regulation of cell-cell adhesion; ephrin receptor signaling pathway; angiogenesis; urogenital system development; retinal ganglion cell axon guidance