Western Blot (WB)
((1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EPHB2/DRT/Nuk Polyclonal Antibody | anti-EPHB2 antibody
Goat anti-EPHB2/DRT/Nuk Antibody
Western Blot: Approx 60kDa band observed in Human Lung and Placenta lysates (calculated MW of 110kDa according to NP_059145.2 and NP_004433.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Litterst et al, J Biol Chem.2007 Apr 10; PMID: 17428795). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
Uniprot Description
EphB2: a receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members. Activated EphB2 recruits RasGAP, down-regulating the Ras-Erk signaling axis and neurite retraction. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB2 interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Point mutations seen in prostate cancer. Overexpressed and required for migration of glioblastoma. Overexpressed and correlated with poor survival in breast cancer. Overexpression and loss of heterozygosity seen in colorectal cancers. Target for immunoconjugate drug therapy .Three splice-variant isoforms have been described.
Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Tumor suppressor; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; Eph family
Chromosomal Location of Human Ortholog: 1p36.1-p35
Cellular Component: axon; cell soma; cytosol; dendrite; extracellular region; integral to plasma membrane; plasma membrane
Molecular Function: ATP binding; axon guidance receptor activity; identical protein binding; protein binding; protein-tyrosine kinase activity; receptor binding; transmembrane-ephrin receptor activity
Biological Process: angiogenesis; axon guidance; axonal fasciculation; camera-type eye morphogenesis; central nervous system projection neuron axonogenesis; corpus callosum development; ephrin receptor signaling pathway; inner ear morphogenesis; learning; negative regulation of axonogenesis; nervous system development; optic nerve morphogenesis; palate development; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of long-term neuronal synaptic plasticity; positive regulation of synaptogenesis; regulation of body fluid levels; retinal ganglion cell axon guidance; urogenital system development
Disease: Prostate Cancer/brain Cancer Susceptibility
Research Articles on EPHB2
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Product Notes
The EPHB2 ephb2 (Catalog #AAA420858) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EPHB2/DRT/Nuk Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's EPHB2/DRT/Nuk can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 60kDa band observed in Human Lung and Placenta lysates (calculated MW of 110kDa according to NP_059145.2 and NP_004433.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Litterst et al, J Biol Chem.2007 Apr 10; PMID: 17428795). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the EPHB2 ephb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QKDRNHRPKF GQ. It is sometimes possible for the material contained within the vial of "EPHB2/DRT/Nuk, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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