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Immunohistochemistry (IHC) (Eph receptor A6 (EPHA6) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Eph receptor A6 (EPHA6) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Rabbit anti-Human, Mouse EphA6 Polyclonal Antibody | anti-EphA6 antibody

EphA6, CT (Ephrin Type-A Receptor 6, EPH Homology Kinase 2, EHK-2, Ehk-2, Ehk2) (PE)

Gene Names
EPHA6; EHK2; EK12; EPA6; EHK-2; HEK12; PRO57066
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
EphA6; Polyclonal Antibody; CT (Ephrin Type-A Receptor 6; EPH Homology Kinase 2; EHK-2; Ehk-2; Ehk2) (PE); anti-EphA6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human EphA6. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-EphA6 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of mouse EphA6 (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Eph receptor A6 (EPHA6) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Eph receptor A6 (EPHA6) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Eph receptor A6 (EPHA6) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Eph receptor A6 (EPHA6) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of Eph receptor A6 (EPHA6) Antibody (C-term) in mouse stomach tissue lysates (35ug/lane). EPHA6 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of Eph receptor A6 (EPHA6) Antibody (C-term) in mouse stomach tissue lysates (35ug/lane). EPHA6 (arrow) was detected using the purified Pab.)
Product Categories/Family for anti-EphA6 antibody
References
1. Lee, A.M., et al., DNA Cell Biol. 15(10):817-825 (1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
116,116 Da
NCBI Official Full Name
ephrin type-A receptor 6 isoform b
NCBI Official Synonym Full Names
EPH receptor A6
NCBI Official Symbol
EPHA6
NCBI Official Synonym Symbols
EHK2; EK12; EPA6; EHK-2; HEK12; PRO57066
NCBI Protein Information
ephrin type-A receptor 6; EPH-like kinase 12; EPH homology kinase 2; ephrin receptor EphA6
UniProt Protein Name
Ephrin type-A receptor 6
Protein Family
UniProt Gene Name
EPHA6
UniProt Synonym Gene Names
EHK2; HEK12; EHK-2; EK12
UniProt Entry Name
EPHA6_HUMAN

Uniprot Description

EphA6: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; Membrane protein, integral; TK group; Eph family

Chromosomal Location of Human Ortholog: 3q11.2

Cellular Component: nucleoplasm; integral to plasma membrane; plasma membrane

Molecular Function: ephrin receptor activity; ATP binding

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; ephrin receptor signaling pathway

Research Articles on EphA6

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Product Notes

The EphA6 epha6 (Catalog #AAA6474299) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EphA6, CT (Ephrin Type-A Receptor 6, EPH Homology Kinase 2, EHK-2, Ehk-2, Ehk2) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EphA6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EphA6 epha6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EphA6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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