Rabbit anti-Human, Mouse EphA5 Polyclonal Antibody | anti-EphA5 antibody

EphA5, NT (Ephrin type-A receptor 5, Tyrosine-protein kinase receptor EHK-1, Eph homology kinase-1, Receptor protein-tyrosine kinase HEK7, EPH-like kinase 7, EK7, EHK1, HEK7) (Biotin)

Cat. Num. AAA6474258

• Gene Names: EPHA5; CEK7; EHK1; HEK7; TYRO4
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: EphA5; Polyclonal Antibody; NT (Ephrin type-A receptor 5; Tyrosine-protein kinase receptor EHK-1; Eph homology kinase-1; Receptor protein-tyrosine kinase HEK7; EPH-like kinase 7; EK7; EHK1; HEK7) (Biotin); anti-EphA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphA5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EphA5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human EphA5 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-EphA5 N-term Pab in mouse brain tissue. EphA5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with EphA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-EphA5 antibody

Antibodies; Protein Kinases

References

1. Pejovic, T. et al., Transl Oncol. Dec 01; 2(4): 341-9(2009). 2. Fox, G.M., et al., Oncogene 10(5):897-905 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 221625401
• NCBI GeneID: 2044
• NCBI Accession #: NP_004430.4
• NCBI GenBank Nucleotide #: NM_004439.5
• UniProt Accession #: P54756; Q7Z3F2
• Molecular Weight: 114,803 Da
• NCBI Official Full Name: ephrin type-A receptor 5 isoform a
• NCBI Official Synonym Full Names: EPH receptor A5
• NCBI Official Symbol: EPHA5
• NCBI Official Synonym Symbols: CEK7; EHK1; HEK7; TYRO4
• NCBI Protein Information: ephrin type-A receptor 5; EK7; EHK-1; EPH-like kinase 7; EPH homology kinase 1; Eph homology kinase-1; brain-specific kinase; receptor protein-tyrosine kinase HEK7; tyrosine-protein kinase receptor EHK-1
• UniProt Protein Name: Ephrin type-A receptor 5
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA5
• UniProt Synonym Gene Names: BSK; EHK1; HEK7; TYRO4; EHK-1; EK7; hEK7
• UniProt Entry Name: EPHA5_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

EphA5: Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 4q13.1

Cellular Component: rough endoplasmic reticulum; cell soma; axon; perinuclear region of cytoplasm; integral to plasma membrane; dendrite; plasma membrane; external side of plasma membrane

Molecular Function: transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; ephrin receptor activity; ATP binding

Biological Process: axon guidance; cAMP-mediated signaling; peptidyl-tyrosine phosphorylation; regulation of actin cytoskeleton organization and biogenesis; hippocampus development; activation of CREB transcription factor; ephrin receptor signaling pathway; neuron development